Cytoplasmic and nuclear uptake of aldosterone in toad bladder: a mathematical modeling approach

Details

Serval ID
serval:BIB_296F0326F222
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cytoplasmic and nuclear uptake of aldosterone in toad bladder: a mathematical modeling approach
Journal
American Journal of Physiology
Author(s)
Claire  M., Steimer  J. L., Oblin  M. E., Gaeggeler  H. P., Venot  A., Corvol  P., Rossier  B. C.
ISSN
0363-6143
Publication state
Published
Issued date
01/1985
Volume
248
Number
1 Pt 1
Pages
C88-101
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan
Abstract
The mechanism of aldosterone uptake in the epithelial cells of toad bladder was studied using mathematical modeling. Two complementary approaches were used. The first involved analysis of cytosolic aldosterone binding at steady state according to models defined by the sum of independent noninteractive binding sites. The best model describing the experimental data corresponded to two specific binding sites with mean dissociation constant values of 0.20 and 60 nM for types 1 and 2, respectively. The second approach was based on the analysis of cytoplasmic and nuclear aldosterone uptake kinetics at 25 and 0 degrees C in intact bladder. Two models (A and B) were studied. They both implied the existence of two types of aldosterone binding sites as precursors of the corresponding chromatin bound complexes. In model A, nuclear translocation of the two types of receptors was assumed to obey first-order kinetics. In model B, the translocation process for type 1 sites involved a time lag leading to delayed binding to chromatin. Both models were found to fit the experimental data satisfactorily. The fit obtained for model B appeared to be better at low aldosterone concentrations.
Keywords
Aldosterone/*metabolism Animals Bufo marinus Cell Nucleus/*metabolism Cytosol/metabolism Female Kinetics Male Mathematics Models, Biological Receptors, Aldosterone Receptors, Glucocorticoid/*metabolism Receptors, Steroid/*metabolism Urinary Bladder/*metabolism
Pubmed
Web of science
Create date
24/01/2008 13:01
Last modification date
20/08/2019 13:09
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