Monoclonal anti-MAGE-3 CTL responses in melanoma patients displaying tumor regression after vaccination with a recombinant canarypox virus.

Details

Serval ID
serval:BIB_29592
Type
Article: article from journal or magazin.
Publication sub-type
Case report (case report): feedback on an observation with a short commentary.
Collection
Publications
Institution
Title
Monoclonal anti-MAGE-3 CTL responses in melanoma patients displaying tumor regression after vaccination with a recombinant canarypox virus.
Journal
Journal of Immunology
Author(s)
Karanikas V., Lurquin C., Colau D., van Baren N., De Smet C., Lethé B., Connerotte T., Corbière V., Demoitié M.A., Liénard D., Dréno B., Velu T., Boon T., Coulie P.G.
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Publication state
Published
Issued date
2003
Volume
171
Number
9
Pages
4898-4904
Language
english
Notes
Publication types: Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
We have analyzed the T cell responses of HLA-A1 metastatic melanoma patients with detectable disease, following vaccination with a recombinant ALVAC virus, which bears short MAGE-1 and MAGE-3 sequences coding for antigenic peptides presented by HLA-A1. To evaluate the anti-MAGE CTL responses, we resorted to antigenic stimulation of blood lymphocytes under limiting dilution conditions, followed by tetramer analysis and cloning of the tetramer-positive cells. The clones were tested for their specific lytic ability and their TCR sequences were obtained. Four patients who showed tumor regression were analyzed, and an anti-MAGE-3.A1 CTL response was observed in three of these patients. Postvaccination frequencies of anti-MAGE-3.A1 CTL were 3 x 10(-6), 3 x 10(-3), and 3 x 10(-7) of the blood CD8 T cells, respectively. These three responses were monoclonal. No anti-MAGE-1.A1 CTL response was observed. These results indicate that, like peptide immunization, ALVAC immunization produces monoclonal responses. They also suggest that low-level antivaccine CTL responses can initiate a tumor regression process. Taken together, our analysis of anti-MAGE-3.A1 T cell responses following peptide or ALVAC vaccination shows a degree of correlation between CTL response and tumor regression, but firm conclusions will require larger numbers.
Keywords
Antigens, Neoplasm/administration & dosage, Antigens, Neoplasm/genetics, Canarypox virus/genetics, Canarypox virus/immunology, Cancer Vaccines/administration & dosage, Cancer Vaccines/genetics, Clone Cells, Female, Humans, Injections, Intradermal, Injections, Subcutaneous, Lymphatic Metastasis/immunology, Lymphatic Metastasis/prevention & control, Melanoma/immunology, Melanoma/prevention & control, Neoplasm Proteins/administration & dosage, Neoplasm Proteins/genetics, Polymerase Chain Reaction, Receptors, Antigen, T-Cell/biosynthesis, Receptors, Antigen, T-Cell/blood, T-Lymphocytes, Cytotoxic/immunology, T-Lymphocytes, Cytotoxic/metabolism, Vaccines, Subunit/administration & dosage, Vaccines, Subunit/genetics, Vaccines, Synthetic/administration & dosage, Vaccines, Synthetic/genetics, Viral Vaccines/administration & dosage, Viral Vaccines/genetics
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:27
Last modification date
20/08/2019 13:09
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