Improved immunogenicity against HIV-1 clade C antigens by using NYVAC-C with restored replication competence

Details

Serval ID
serval:BIB_2937B153B472
Type
Inproceedings: an article in a conference proceedings.
Publication sub-type
Abstract (Abstract): shot summary in a article that contain essentials elements presented during a scientific conference, lecture or from a poster.
Collection
Publications
Institution
Title
Improved immunogenicity against HIV-1 clade C antigens by using NYVAC-C with restored replication competence
Title of the conference
AIDS Vaccine 2010
Author(s)
Mooij P., Nieuwenhuis I., Beenhakker N., Hofman S., Esteban M., Jacobs B., Kibler K., Koopman G., Bogers W., Pantaleo G., Heeney J.
Address
Atlanta, Georgia, September 28-October 1, 2010
ISBN
0889-2229
Publication state
Published
Issued date
2010
Peer-reviewed
Oui
Volume
26
Series
Aids Research And Human Retroviruses
Pages
A4-A5
Language
english
Notes
Meeting Abstract
Abstract
Background: In order to improve the immunogenicity of currently available non-replicating pox virus HIV vaccine vectors, NYVAC was genetically modified through re-insertion of two host range genes (K1L and C7L), resulting in restored replicative capacity in human cells.
Methods: In the present study these vectors, expressing either a combination of the HIV-1 clade C antigens Env, Gag, Pol, Nef, or a combination of Gal, Pol, Nef were evaluated for safety and immunogenicity in rhesus macaques, which were immunized at weeks 0, 4 and 12 either by scarification (conventional poxvirus route of immunization), intradermal or by intramuscular injection (route used in previous vaccine studies).
Results: Replication competent NYVAC-C-KC vectors induced higher HIV-specific responses, as measured by IFN-g ELISpot assay, than the replication defective NYVAC-C vectors. Application through scarification only required one immunization to induce maximum HIV-specific immune responses. This method simultaneously induced relatively lower anti-vector responses. In contrast, two to three immunizations were required when the NYVAC-C-KC vectors were given by intradermal or intramuscular injection and this method tended to generate slightly lower responses. Responses were predominantly directed against Env in the animals that received NYVAC-C-KC vectors expressing HIV-1 Env, Gag, Pol, Nef, while Gag responses were dominant in the NYVAC-C-KC HIV-1 Gag, Pol, Nef immunized animals.
Conclusion: The current study demonstrates that NYVAC replication competent vectors were well tolerated and showed increased immunogenicity as compared to replication defective vectors. Further studies are needed to evaluate the most efficient route of immunization and to explore the use of these replication competent
NYVAC vectors in prime/boost combination with gp120 proteinbased vaccine candidates. This study was performed within the Poxvirus T-cell Vaccine Discovery Consortium (PTVDC) which is part of the CAVD program.
Keywords
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Web of science
Create date
20/01/2011 11:53
Last modification date
20/08/2019 13:08
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