Production of low phytic acid rice by hairpin RNA- and artificial microRNA-mediated silencing of OsMIK in seeds

Details

Serval ID
serval:BIB_29277AC22E86
Type
Article: article from journal or magazin.
Collection
Publications
Title
Production of low phytic acid rice by hairpin RNA- and artificial microRNA-mediated silencing of OsMIK in seeds
Journal
Plant Cell Tissue and Organ Culture
Author(s)
Li W.-X., Huang J.-Z., Zhao H.-J., Tan Y.-Y., Cui H.-R., Poirier Y., Shu Q.-Y.
ISSN
1573-5044 (electronic)
ISSN-L
0167-6857
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
119
Number
1
Pages
15-25
Language
english
Abstract
To produce agronomically competitive rice with nutritionally superior, environmentally safe phytic acid (PA) levels, hairpin RNA (hpRNA)- and artificial microRNA (amiRNA)-mediated gene silencing approaches were explored to reduce both myo-inositol kinase gene (OsMIK) expression and PA accumulation in rice seeds. hpRNA and amiRNA sequences targeted to OsMIK (hpMIK and amiMIK), under the control of a rice Ole18 promoter, were transformed into the rice cultivar Nippon-bare. Fourteen and 21 independent transgenic events were identified containing the hpMIK and amiMIK constructs, respectively, from which five stable homozygous transgenic lines of each were developed together with their null siblings. Southern blotting demonstrated transgene integration into the genome and quantitative real-time PCR showed that gene silencing was restricted to seeds. OsMIK transcripts were significantly reduced in both transgenic amiMIK and hpMIK seeds, which had PA levels reduced by 14.9-50.2 and 38.1-50.7 %, respectively, compared with their respective null siblings. There were no systematic significant differences in agronomic traits between the transgenic lines and their non-transgenic siblings, and no correlation between seed PA contents and decreased rates of seed germination and seedling emergence. The results of the present study suggest that Ole 18-driven OsMIK silencing via hpRNA and amiRNA could be an effective way to develop agronomically competitive low phytic acid rice.
Keywords
Artificial microRNA, Seed-specific promoter Ole18, Low phytic acid (LPA), Seed viability, OsMIK, Oryza sativa L
Web of science
Create date
28/10/2014 12:53
Last modification date
20/08/2019 14:08
Usage data