Caulobacter crescentus CdnL is a non-essential RNA polymerase-binding protein whose depletion impairs normal growth and rRNA transcription.

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State: Public
Version: Final published version
Serval ID
serval:BIB_28E1FCA43FE0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Caulobacter crescentus CdnL is a non-essential RNA polymerase-binding protein whose depletion impairs normal growth and rRNA transcription.
Journal
Scientific reports
Author(s)
Gallego-García A., Iniesta A.A., González D., Collier J., Padmanabhan S., Elías-Arnanz M.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
24/02/2017
Peer-reviewed
Oui
Volume
7
Pages
43240
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
CdnL is an essential RNA polymerase (RNAP)-binding activator of rRNA transcription in mycobacteria and myxobacteria but reportedly not in Bacillus. Whether its function and mode of action are conserved in other bacteria thus remains unclear. Because virtually all alphaproteobacteria have a CdnL homolog and none of these have been characterized, we studied the homolog (CdnL <sub>Cc</sub> ) of the model alphaproteobacterium Caulobacter crescentus. We show that CdnL <sub>Cc</sub> is not essential for viability but that its absence or depletion causes slow growth and cell filamentation. CdnL <sub>Cc</sub> is degraded in vivo in a manner dependent on its C-terminus, yet excess CdnL <sub>Cc</sub> resulting from its stabilization did not adversely affect growth. We find that CdnL <sub>Cc</sub> interacts with itself and with the RNAP β subunit, and localizes to at least one rRNA promoter in vivo, whose activity diminishes upon depletion of CdnL <sub>Cc</sub> . Interestingly, cells expressing CdnL <sub>Cc</sub> mutants unable to interact with the RNAP were cold-sensitive, suggesting that CdnL <sub>Cc</sub> interaction with RNAP is especially required at lower than standard growth temperatures in C. crescentus. Our study indicates that despite limited sequence similarities and regulatory differences compared to its myco/myxobacterial homologs, CdnL <sub>Cc</sub> may share similar biological functions, since it affects rRNA synthesis, probably by stabilizing open promoter-RNAP complexes.
Keywords
Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Caulobacter crescentus/genetics, Caulobacter crescentus/metabolism, DNA-Directed RNA Polymerases/metabolism, Gene Expression Regulation, Bacterial, Protein Interaction Domains and Motifs, RNA, Ribosomal/metabolism, RNA-Binding Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
27/01/2017 18:07
Last modification date
20/08/2019 14:08
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