Yeast telomerase is specialized for C/A-rich RNA templates.

Details

Serval ID
serval:BIB_28437
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Yeast telomerase is specialized for C/A-rich RNA templates.
Journal
Nucleic Acids Research
Author(s)
Förstemann K., Zaug A.J., Cech T.R., Lingner J.
ISSN
1362-4962[electronic]
Publication state
Published
Issued date
2003
Volume
31
Number
6
Pages
1646-1655
Language
english
Notes
Publication types: Journal Article
Abstract
Telomeres, the protective caps of eukaryotic chromosomes, are maintained by the enzyme telomerase. This telomere-specific reverse transcriptase (RT) uses a small region of its RNA subunit as template to synthesize telomeric DNA, which is generally G/T rich in the strand that contains the 3' end. To further our understanding of why telomeres are usually G/T rich, we screened Saccharomyces cerevisiae telomerase RNA (TLC1) libraries with randomized template sequences for complementation of a tlc1 deletion and decapping of existing telomeres. Surprisingly, the vast majority of the 60 000 different mutant telomerase templates tested showed no activity in vivo. This deficiency was not due to impaired assembly with the catalytic subunit (Est2p) nor could it be alleviated by enforced telomerase recruitment to the telomeres. Rather, the mutant templates reduced the nucleotide addition processivity of telomerase. The functional RNA template sequences recovered in our screens preferentially contained two or more consecutive rC nucleotides, reminiscent of the wild-type template. Thus, in contrast to retroviral RTs that can reverse transcribe any RNA sequence into DNA, the budding yeast telomerase RT is specialized for its C-rich RNA template.
Keywords
Adenine/metabolism, Base Sequence, Cytosine/metabolism, Genetic Complementation Test, Mutation, Protein Binding, RNA, Fungal/genetics, RNA, Fungal/metabolism, Saccharomyces cerevisiae/enzymology, Saccharomyces cerevisiae/genetics, Substrate Specificity, Telomerase/genetics, Telomerase/metabolism, Telomere/genetics, Telomere/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:25
Last modification date
20/08/2019 13:07
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