Escherichia coli endotoxin reduces cytochrome aa3 redox status in pig skeletal muscle

Details

Serval ID
serval:BIB_283D76D3D43F
Type
Article: article from journal or magazin.
Collection
Publications
Title
Escherichia coli endotoxin reduces cytochrome aa3 redox status in pig skeletal muscle
Journal
Crit Care Med
Author(s)
Forget A. P., Mangalaboyi J., Mordon S., Guery B., Vallet B., Fourrier F., Chopin C.
ISSN
0090-3493 (Print)
ISSN-L
0090-3493
Publication state
Published
Issued date
10/2000
Volume
28
Number
10
Pages
3491-7
Language
english
Notes
Forget, A P
Mangalaboyi, J
Mordon, S
Guery, B
Vallet, B
Fourrier, F
Chopin, C
eng
Research Support, Non-U.S. Gov't
Crit Care Med. 2000 Oct;28(10):3491-7. doi: 10.1097/00003246-200010000-00022.
Abstract
OBJECTIVE: To assess the effect of endotoxin on cytochrome aa3 (Caa3) redox status in a controlled blood flow preparation of pig isolated hindlimb, at a constant oxygen delivery (Do2limb) (constant flow period) and during progressive ischemia (decreasing flow period). DESIGN: Randomized, controlled experimental study. SETTING: University hospital experimental laboratory. SUBJECTS: Ten piglets. INTERVENTIONS: Hindlimb blood flow was restricted to the femoral vessels. The arterial femoral blood flow coming from the carotid artery was controlled by a roller occlusive pump. The femoral venous blood flow was returned to the jugular vein. During the first 100 mins, the hindlimb blood flow was maintained at a normal level and then decreased stepwise. Animals were randomized to receive 150 microg/kg endotoxin lipopolysaccharide (LPS; n = 5) or saline (control; n = 5). MEASUREMENTS AND MAIN RESULTS: Hindlimb muscle Caa3 redox status was monitored by near-infrared spectroscopy. Hindlimb Do2limb and oxygen consumption (Vo2limb) were calculated. In the LPS group, a rapid reduction of Caa3 redox status was observed after LPS administration, whereas the hindlimb blood flow remained normal with no change in Do2limb and Vo2limb. A progressive simultaneous decrease in Do2limb and Vo2limb was observed during the decreasing flow period with no further reduction in Caa3 redox status. In the control group, no change was observed in Caa3, Do2limb, or Vo2limb during the constant flow period. During the decreasing flow period, Caa3 redox status was reduced as Do2limb and Vo2limb decreased. CONCLUSION: Our results suggest that endotoxin may induce a reduction of Caa3 redox status independently of Do2 and Vo2.
Keywords
Animals, Disease Models, Animal, Electron Transport Complex IV/immunology/*metabolism, *Escherichia coli, Female, Hemodynamics, Hindlimb, Inflammation, Linear Models, Lipopolysaccharides/*adverse effects, Monitoring, Physiologic, Multiple Organ Failure/*etiology/immunology/*metabolism/physiopathology, Muscle, Skeletal/*blood supply/*enzymology/immunology, Oxidation-Reduction, *Oxygen Consumption, Random Allocation, Shock, Septic/*complications/immunology/*metabolism/physiopathology, Spectroscopy, Near-Infrared, Swine
Pubmed
Create date
29/04/2021 10:59
Last modification date
30/04/2021 6:38
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