Inhibition of lymphocyte protease granzyme A by antithrombin III
Details
Serval ID
serval:BIB_282BECCC10D3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Inhibition of lymphocyte protease granzyme A by antithrombin III
Journal
Molecular Immunology
ISSN
0161-5890 (Print)
Publication state
Published
Issued date
12/1988
Volume
25
Number
12
Pages
1283-9
Notes
Journal Article --- Old month value: Dec
Abstract
T-lymphocytes contain a cytoplasmic granule associated homo-dimeric protease designated granzyme A. Upon T-cell target cell interaction, the granules undergo exocytosis and granzyme A, and other granule constituents, are released. Here we show that granzyme A secreted into plasma is immediately inactivated by antithrombin III. The rate of complex formation is enhanced 400-fold in the presence of heparin. Two different complexes are generated: granzyme A-antithrombin III and granzyme A-(antithrombin III)2, respectively, indicating that both active centers of granzyme A are functional. Thus, the proteolytic activity of lymphocyte protease granzyme A, whose physiologically relevant function is unknown, is well regulated in plasma.
Keywords
Animals
Antithrombin III/*pharmacology
Blood
Cells, Cultured
Granzymes
Heparin/pharmacology
Kinetics
Mice
*Serine Endopeptidases
*Serine Proteinase Inhibitors
T-Lymphocytes, Cytotoxic/*enzymology
Pubmed
Web of science
Create date
24/01/2008 15:18
Last modification date
20/08/2019 13:07