High frequencies of functionally impaired cytokeratin 18-specific CD8+ T cells in healthy HLA-A2+ donors.

Details

Serval ID
serval:BIB_280BD97AE7DE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
High frequencies of functionally impaired cytokeratin 18-specific CD8+ T cells in healthy HLA-A2+ donors.
Journal
European Journal of Immunology
Author(s)
Walter S., Bioley G., Bühring H.J., Koch S., Wernet D., Zippelius A., Pawelec G., Romero P., Stevanović S., Rammensee H.G., Gouttefangeas C.
ISSN
0014-2980, 0014-2980[linking]
Publication state
Published
Issued date
2005
Volume
35
Number
10
Pages
2876-2885
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Combining cell surface phenotyping with functional analysis, human CD8+ T cells have been divided into several subsets which are being studied extensively in diverse physiological situations, such as viral infection, cancer and ageing. In particular, so-called terminally differentiated effector cells possess a CD45RA+ CCR7- CD27- CD28- phenotype, contain perforin and, in different models, have been shown to exert direct ex vivo killing and to release interleukins upon both antigen-nonspecific and -specific stimulation. Using HLA class I multimers, we have identified a high frequency of peripheral CD8+ T cells that recognize a peptide derived from the self protein cytokeratin 18 presented by the HLA-A*0201 molecule. These cells can be detected in approximately 15% of the HLA-A2-positive healthy donors tested. A detailed analysis revealed that they must have divided extensively in vivo, have an effector cell phenotype and express various natural killer cell-associated receptors. Interestingly, however, they remained unresponsive to antigen-specific stimulation in vitro in terms of cytotoxicity and cytokine secretion. Thus, cytokeratin 18-specific cells constitute a frequently encountered, new CD8+ T lymphocyte subpopulation without classical effector status and with so far unknown function.
Keywords
CD8-Positive T-Lymphocytes/cytology, CD8-Positive T-Lymphocytes/immunology, Flow Cytometry, HLA-A2 Antigen/immunology, HLA-A2 Antigen/metabolism, Humans, Immunophenotyping, Keratins/immunology, Keratins/metabolism, Phenotype, T-Lymphocyte Subsets/immunology, T-Lymphocyte Subsets/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:28
Last modification date
20/08/2019 13:07
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