Transcriptional alterations in the left ventricle of three hypertensive rat models.

Details

Serval ID
serval:BIB_2807891D81AC
Type
Article: article from journal or magazin.
Collection
Publications
Title
Transcriptional alterations in the left ventricle of three hypertensive rat models.
Journal
Physiological Genomics
Author(s)
Cerutti C., Kurdi M., Bricca G., Hodroj W., Paultre C., Randon J., Gustin M.P.
ISSN
1531-2267[electronic]
Publication state
Published
Issued date
2006
Volume
27
Number
3
Pages
295-308
Language
english
Abstract
Left ventricular hypertrophy (LVH) is commonly associated with hypertension and represents an independent cardiovascular risk factor. The aim of this study was to test the hypothesis that the cardiac overload related to hypertension is associated to a specific gene expression pattern independently of genetic background. Gene expression levels were obtained with microarrays for 15,866 transcripts from RNA of left ventricles from 12-wk-old rats of three hypertensive models [spontaneously hypertensive rat (SHR), Lyon hypertensive rat (LH), and heterozygous TGR(mRen2)27 rat] and their respective controls. More than 60% of the detected transcripts displayed significant changes between the three groups of normotensive rats, showing large interstrain variability. Expression data were analyzed with respect to hypertension, LVH, and chromosomal distribution. Only four genes had significantly modified expression in the three hypertensive models among which a single gene, coding for sialyltransferase 7A, was consistently overexpressed. Correlation analysis between expression data and left ventricular mass index (LVMI) over all rats identified a larger set of genes whose expression was continuously related with LVMI, including known genes associated with cardiac remodeling. Positioning the detected transcripts along the chromosomes pointed out high-density regions mostly located within blood pressure and cardiac mass quantitative trait loci. Although our study could not detect a unique reprogramming of cardiac cells involving specific genes at early stage of LVH, it allowed the identification of some genes associated with LVH regardless of genetic background. This study thus provides a set of potentially important genes contained within restricted chromosomal regions involved in cardiovascular diseases.
Keywords
Animals, Animals, Genetically Modified, Gene Expression Profiling, Heart Ventricles/metabolism, Heart Ventricles/pathology, Hypertension/genetics, Hypertrophy, Left Ventricular/genetics, Male, Mice, Oligonucleotide Array Sequence Analysis, Rats, Rats, Inbred SHR, Rats, Inbred WKY, Renin/genetics, Sialyltransferases/genetics, Species Specificity, Transcription, Genetic
Pubmed
Web of science
Create date
18/09/2008 16:40
Last modification date
20/08/2019 13:07
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