Cutting edge: influence of the TCR V beta domain on the avidity of CD1d:alpha-galactosylceramide binding by invariant V alpha 14 NKT cells.
Details
Serval ID
serval:BIB_28040
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cutting edge: influence of the TCR V beta domain on the avidity of CD1d:alpha-galactosylceramide binding by invariant V alpha 14 NKT cells.
Journal
Journal of immunology
ISSN
0022-1767
Publication state
Published
Issued date
2003
Peer-reviewed
Oui
Volume
170
Number
12
Pages
5815-5819
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
CD1d tetramers loaded with alpha-galactosylceramide (alpha-GalCer) bind selectively to mouse invariant Valpha14 (Valpha14i) NKT cells and their human counterparts. Whereas tetramer binding strictly depends on the expression of a Valpha14-Jalpha18 chain in murine NKT cells, the associated beta-chain (typically expressing Vbeta8.2 or Vbeta7) appears not to influence tetramer binding. In this study, we describe novel alpha-GalCer-loaded mouse and human CD1d-IgG1 dimers, which revealed an unexpected influence of the TCR-beta chain on the avidity of CD1d:alpha-GalCer binding. A subset of Valpha14i NKT cells clearly discriminated alpha-GalCer bound to mouse or human CD1d on the basis of avidity differences conferred by the Vbeta domain of the TCR-beta chain, with Vbeta8.2 conferring higher avidity binding than Vbeta7.
Keywords
Animals, Antigens, CD1/metabolism, Antigens, CD1d, Cell Adhesion/immunology, Dimerization, Dose-Response Relationship, Immunologic, Galactosylceramides/metabolism, Humans, Killer Cells, Natural/immunology, Killer Cells, Natural/metabolism, Mice, Mice, Inbred C57BL, Peptide Fragments/physiology, Protein Structure, Tertiary, Receptors, Antigen, T-Cell, alpha-beta/physiology, T-Lymphocyte Subsets/immunology, T-Lymphocyte Subsets/metabolism, Tumor Cells, Cultured
OAI-PMH
Pubmed
Web of science
Create date
19/11/2007 13:24
Last modification date
20/08/2019 14:07
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