Closely related dermatophyte species produce different patterns of secreted proteins.

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Serval ID
serval:BIB_27D353F4CFF3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Closely related dermatophyte species produce different patterns of secreted proteins.
Journal
Fems Microbiology Letters
Author(s)
Giddey K., Favre B., Quadroni M., Monod M.
ISSN
0378-1097 (Print)
ISSN-L
0378-1097
Publication state
Published
Issued date
02/2007
Peer-reviewed
Oui
Volume
267
Number
1
Pages
95-101
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Dermatophytes are the most common infectious agents responsible for superficial mycosis in humans and animals. Various species in this group of fungi show overlapping characteristics. We investigated the possibility that closely related dermatophyte species with different behaviours secrete distinct proteins when grown in the same culture medium. Protein patterns from culture filtrates of several strains of the same species were very similar. In contrast, secreted protein profiles from various species were different, and so a specific signature could be associated with each of the six analysed species. In particular, protein patterns were useful to distinguish Trichophyton tonsurans from Trichophyton equinum, which cannot be differentiated by ribosomal DNA sequencing. The secreted proteases Sub2, Sub6 and Sub7 of the subtilisin family, as well as Mep3 and Mep4 of the fungalisin family were identified. SUB6, SUB7, MEP3 and MEP4 genes were cloned and sequenced. Although the protein sequence of each protease was highly conserved across species, their level of secretion by the various species was not equivalent. These results suggest that a switch of habitat could be related to a differential expression of genes encoding homologous secreted proteins.
Keywords
Amino Acid Sequence, Arthrodermataceae/classification, Arthrodermataceae/genetics, Conserved Sequence, Culture Media/chemistry, DNA, Fungal/chemistry, DNA, Fungal/isolation & purification, DNA, Ribosomal/chemistry, DNA, Ribosomal/isolation & purification, Electrophoresis, Polyacrylamide Gel, Fungal Proteins/genetics, Fungal Proteins/isolation & purification, Genes, rRNA, Molecular Sequence Data, Peptide Hydrolases/chemistry, Peptide Hydrolases/metabolism, Protein Transport, Proteome/analysis, RNA, Fungal/genetics, RNA, Ribosomal/genetics, Sequence Analysis, DNA
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 16:46
Last modification date
25/09/2019 7:08
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