Essential Bacillus subtilis genes.

Details

Serval ID
serval:BIB_27965
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Essential Bacillus subtilis genes.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Kobayashi K., Ehrlich S.D., Albertini A., Amati G., Andersen K.K., Arnaud M., Asai K., Ashikaga S., Aymerich S., Bessieres P., Boland F., Brignell S.C., Bron S., Bunai K., Chapuis J., Christiansen L.C., Danchin A., Débarbouille M., Dervyn E., Deuerling E., Devine K., Devine S.K., Dreesen O., Errington J., Fillinger S., Foster S.J., Fujita Y., Galizzi A., Gardan R., Eschevins C., Fukushima T., Haga K., Harwood C.R., Hecker M., Hosoya D., Hullo M.F., Kakeshita H., Karamata D., Kasahara Y., Kawamura F., Koga K., Koski P., Kuwana R., Imamura D., Ishimaru M., Ishikawa S., Ishio I., Le Coq D., Masson A., Mauël C., Meima R., Mellado R.P., Moir A., Moriya S., Nagakawa E., Nanamiya H., Nakai S., Nygaard P., Ogura M., Ohanan T., O'Reilly M., O'Rourke M., Pragai Z., Pooley H.M., Rapoport G., Rawlins J.P., Rivas L.A., Rivolta C., Sadaie A., Sadaie Y., Sarvas M., Sato T., Saxild H.H., Scanlan E., Schumann W., Seegers J.F., Sekiguchi J., Sekowska A., Séror S.J., Simon M., Stragier P., Studer R., Takamatsu H., Tanaka T., Takeuchi M., Thomaides H.B., Vagner V., van Dijl J.M., Watabe K., Wipat A., Yamamoto H., Yamamoto M., Yamamoto Y., Yamane K., Yata K., Yoshida K., Yoshikawa H., Zuber U., Ogasawara N.
ISSN
0027-8424[print], 0027-8424[linking]
Publication state
Published
Issued date
2003
Peer-reviewed
Oui
Volume
100
Number
8
Pages
4678-4683
Language
english
Abstract
To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among approximately 4,100 genes of the organism, only 192 were shown to be indispensable by this or previous work. Another 79 genes were predicted to be essential. The vast majority of essential genes were categorized in relatively few domains of cell metabolism, with about half involved in information processing, one-fifth involved in the synthesis of cell envelope and the determination of cell shape and division, and one-tenth related to cell energetics. Only 4% of essential genes encode unknown functions. Most essential genes are present throughout a wide range of Bacteria, and almost 70% can also be found in Archaea and Eucarya. However, essential genes related to cell envelope, shape, division, and respiration tend to be lost from bacteria with small genomes. Unexpectedly, most genes involved in the Embden-Meyerhof-Parnas pathway are essential. Identification of unknown and unexpected essential genes opens research avenues to better understanding of processes that sustain bacterial life.
Keywords
Bacillus subtilis/cytology, Bacillus subtilis/genetics, Cell Division/genetics, Cell Membrane/genetics, Coenzymes/genetics, Coenzymes/metabolism, Energy Metabolism/genetics, Genes, Bacterial, Genome, Bacterial, Mutation, Nucleotides/genetics, Nucleotides/metabolism, Phylogeny
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:24
Last modification date
20/08/2019 13:06
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