Deletion of hypothetical wall teichoic acid ligases in Staphylococcus aureus activates the cell wall stress response.

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Ressource 1Download: serval:BIB_2734E936DFE5.P001 (603.16 [Ko])
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Serval ID
serval:BIB_2734E936DFE5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Deletion of hypothetical wall teichoic acid ligases in Staphylococcus aureus activates the cell wall stress response.
Journal
FEMS Microbiology Letters
Author(s)
Dengler V., Meier P.S., Heusser R., Kupferschmied P., Fazekas J., Friebe S., Staufer S.B., Majcherczyk P.A., Moreillon P., Berger-Bächi B., McCallum N.
ISSN
1574-6968 (Electronic)
ISSN-L
0378-1097
Publication state
Published
Issued date
2012
Volume
333
Number
2
Pages
109-120
Language
english
Abstract
The Staphylococcus aureus cell wall stress stimulon (CWSS) is activated by cell envelope-targeting antibiotics or depletion of essential cell wall biosynthesis enzymes. The functionally uncharacterized S. aureus LytR-CpsA-Psr (LCP) proteins, MsrR, SA0908 and SA2103, all belong to the CWSS. Although not essential, deletion of all three LCP proteins severely impairs cell division. We show here that VraSR-dependent CWSS expression was up to 250-fold higher in single, double and triple LCP mutants than in wild type S. aureus in the absence of external stress. The LCP triple mutant was virtually depleted of wall teichoic acids (WTA), which could be restored to different degrees by any of the single LCP proteins. Subinhibitory concentrations of tunicamycin, which inhibits the first WTA synthesis enzyme TarO (TagO), could partially complement the severe growth defect of the LCP triple mutant. Both of the latter findings support a role for S. aureus LCP proteins in late WTA synthesis, as in Bacillus subtilis where LCP proteins were recently proposed to transfer WTA from lipid carriers to the cell wall peptidoglycan. Intrinsic activation of the CWSS upon LCP deletion and the fact that LCP proteins were essential for WTA-loading of the cell wall, highlight their important role(s) in S. aureus cell envelope biogenesis.
Keywords
cell wall stress stimulon, LytR-CpsA-Psr, VraSR, wall teichoic acids, ligase, Staphylococcus aureus
Pubmed
Web of science
Open Access
Yes
Create date
27/08/2012 18:15
Last modification date
25/09/2019 7:08
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