FXYD7 is a brain-specific regulator of Na,K-ATPase alpha 1-beta isozymes.

Details

Serval ID
serval:BIB_269BD8440D3F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
FXYD7 is a brain-specific regulator of Na,K-ATPase alpha 1-beta isozymes.
Journal
EMBO Journal
Author(s)
Béguin P., Crambert G., Monnet-Tschudi F., Uldry M., Horisberger J.D., Garty H., Geering K.
ISSN
0261-4189 (Print)
ISSN-L
0261-4189
Publication state
Published
Issued date
2002
Volume
21
Number
13
Pages
3264-3273
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Recently, corticosteroid hormone-induced factor (CHIF) and the gamma-subunit, two members of the FXYD family of small proteins, have been identified as regulators of renal Na,K-ATPase. In this study, we have investigated the tissue distribution and the structural and functional properties of FXYD7, another family member which has not yet been characterized. Expressed exclusively in the brain, FXYD7 is a type I membrane protein bearing N-terminal, post-translationally added modifications on threonine residues, most probably O-glycosylations that are important for protein stabilization. Expressed in Xenopus oocytes, FXYD7 can interact with Na,K-ATPase alpha 1-beta 1, alpha 2-beta 1 and alpha 3-beta 1 but not with alpha-beta 2 isozymes, whereas, in brain, it is only associated with alpha 1-beta isozymes. FXYD7 decreases the apparent K(+) affinity of alpha 1-beta 1 and alpha 2-beta 1, but not of alpha 3-beta1 isozymes. These data suggest that FXYD7 is a novel, tissue- and isoform-specific Na,K-ATPase regulator which could play an important role in neuronal excitability.
Keywords
Action Potentials, Animals, Brain Chemistry, Brefeldin A/pharmacology, DNA, Complementary/genetics, Glycosylation, Isoenzymes/metabolism, Kidney/metabolism, Membrane Glycoproteins/chemistry, Membrane Glycoproteins/genetics, Mice, Microsomes/metabolism, Mutagenesis, Site-Directed, Nerve Tissue Proteins/chemistry, Nerve Tissue Proteins/genetics, Neurons/metabolism, Oocytes, Organ Specificity, Potassium/metabolism, Protein Interaction Mapping, Protein Processing, Post-Translational, Protein Subunits, RNA, Messenger/analysis, Rats, Recombinant Fusion Proteins/physiology, Sodium-Potassium-Exchanging ATPase/genetics, Sodium-Potassium-Exchanging ATPase/metabolism, Structure-Activity Relationship, Xenopus laevis
Pubmed
Web of science
Create date
24/01/2008 12:28
Last modification date
20/08/2019 13:05
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