Blood CXCR3 (+) CD4 T Cells Are Enriched in Inducible Replication Competent HIV in Aviremic Antiretroviral Therapy-Treated Individuals.

Details

Serval ID
serval:BIB_264758601E35
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Blood CXCR3 (+) CD4 T Cells Are Enriched in Inducible Replication Competent HIV in Aviremic Antiretroviral Therapy-Treated Individuals.
Journal
Frontiers in immunology
Author(s)
Banga R., Procopio F.A., Ruggiero A., Noto A., Ohmiti K., Cavassini M., Corpataux J.M., Paxton W.A., Pollakis G., Perreau M.
ISSN
1664-3224 (Print)
ISSN-L
1664-3224
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
9
Pages
144
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
We recently demonstrated that lymph nodes (LNs) PD-1 javax.xml.bind.JAXBElement@6be93b55 /T follicular helper (Tfh) cells from antiretroviral therapy (ART)-treated HIV-infected individuals were enriched in cells containing replication competent virus. However, the distribution of cells containing inducible replication competent virus has been only partially elucidated in blood memory CD4 T-cell populations including the Tfh cell counterpart circulating in blood (cTfh). In this context, we have investigated the distribution of (1) total HIV-infected cells and (2) cells containing replication competent and infectious virus within various blood and LN memory CD4 T-cell populations of conventional antiretroviral therapy (cART)-treated HIV-infected individuals. In the present study, we show that blood CXCR3-expressing memory CD4 T cells are enriched in cells containing inducible replication competent virus and contributed the most to the total pool of cells containing replication competent and infectious virus in blood. Interestingly, subsequent proviral sequence analysis did not indicate virus compartmentalization between blood and LN CD4 T-cell populations, suggesting dynamic interchanges between the two compartments. We then investigated whether the composition of blood HIV reservoir may reflect the polarization of LN CD4 T cells at the time of reservoir seeding and showed that LN PD-1 javax.xml.bind.JAXBElement@21c52b78 CD4 T cells of viremic untreated HIV-infected individuals expressed significantly higher levels of CXCR3 as compared to CCR4 and/or CCR6, suggesting that blood CXCR3-expressing CD4 T cells may originate from LN PD-1 javax.xml.bind.JAXBElement@1041416b CD4 T cells. Taken together, these results indicate that blood CXCR3-expressing CD4 T cells represent the major blood compartment containing inducible replication competent virus in treated aviremic HIV-infected individuals.

Keywords
CXCR3, T follicular helper cells, circulating T follicular helper cell counterpart, lymph node, replication competent virus
Pubmed
Web of science
Create date
27/02/2018 8:17
Last modification date
20/08/2019 13:04
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