Differential expression of the HsKin17 protein during differentiation of in vitro reconstructed human skin.

Details

Serval ID
serval:BIB_263C1785D729
Type
Article: article from journal or magazin.
Collection
Publications
Title
Differential expression of the HsKin17 protein during differentiation of in vitro reconstructed human skin.
Journal
Archives of dermatological research
Author(s)
Biard D.S., Saintigny Y., Maratrat M., Vozenin M.C., Martin M., Daburon F., Angulo J.F.
ISSN
0340-3696 (Print)
ISSN-L
0340-3696
Publication state
Published
Issued date
07/1997
Peer-reviewed
Oui
Volume
289
Number
8
Pages
448-456
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
In eukaryotic cells, various proteins homologous to the E. coli RecA protein are involved in the elimination of DNA damage. These proteins contribute to the repair of double-strand breaks and to genetic recombination. The mouse Kin17 protein is recognised by antibodies directed against the RecA protein. Kin17 has a zinc-finger domain allowing binding to curved DNA stretching over illegitimate recombination junctions. In the present study, we identified the human counterpart of the mouse Kin17 protein (named HsKin17) in skin cells. We employed an in vitro reconstructed skin model composed of an epidermal sheath lying on a dermal matrix with human fibroblasts embedded in rat collagen type I. The maturation programme (proliferation versus differentiation) of keratinocytes was highly dependent on stromal cells. Immunohistochemical staining of frozen sections obtained from skin specimens was monitored by an interactive laser cytometer. In this way we analysed protein levels in both dermal and epidermal compartments. After having characterised the epithelium, we focused our attention on HsKin17 expression. We detected HsKin17 in human keratinocytes. HsKin17 protein levels increased in proliferating epithelial keratinocytes after 7 days of culture. After 2 weeks of culture, epidermal sheaths acquired most of the differentiated features of mature epithelium. At this time, HsKin17 protein dropped below measurable levels in the stratum corneum, and diminished in nucleated cells. This study showed that HsKin17 is expressed in human reconstructed epithelium under conditions of hyperproliferation.

Keywords
Adult, Animals, Basement Membrane/cytology, Cell Differentiation, Cells, Cultured, DNA Repair, DNA-Binding Proteins/analysis, DNA-Binding Proteins/genetics, DNA-Binding Proteins/immunology, Female, Humans, Keratinocytes/chemistry, Mice, Nuclear Proteins, RNA-Binding Proteins, Rats, Skin/chemistry, Skin/cytology
Pubmed
Web of science
Create date
27/04/2018 15:00
Last modification date
20/08/2019 13:04
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