Homology-Independent Integration of Plasmid DNA into the Zebrafish Genome.

Details

Serval ID
serval:BIB_25A5F13371C9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Homology-Independent Integration of Plasmid DNA into the Zebrafish Genome.
Journal
Methods in molecular biology
Author(s)
Auer T.O., Del Bene F.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
1451
Pages
31-51
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Targeting nucleases like zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas) system have revolutionized genome-editing possibilities in many model organisms. They allow the generation of loss-of-function alleles by the introduction of double-strand breaks at defined sites within genes, but also more sophisticated genome-editing approaches have become possible. These include the integration of donor plasmid DNA into the genome by homology-independent repair mechanisms after CRISPR/Cas9-mediated cleavage. Here we present a protocol outlining the most important steps to target a genomic site and to integrate a donor plasmid at this defined locus.

Keywords
Alleles, Animals, CRISPR-Cas Systems/genetics, Clustered Regularly Interspaced Short Palindromic Repeats/genetics, DNA Breaks, Double-Stranded, Genetic Engineering, Plasmids/genetics, Zebrafish/genetics, CRISPR/Cas, Genome editing, Genome engineering, Genome modification, Homology-independent repair, Site-specific nuclease, Targeted transgene integration, Zebrafish
Pubmed
Create date
05/08/2016 18:00
Last modification date
20/08/2019 14:04
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