Migration of centromere proteins in rabbit spermatids
Details
Serval ID
serval:BIB_25595711A314
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Migration of centromere proteins in rabbit spermatids
Journal
Molecular Reproduction and Development
ISSN
1040-452X (Print)
Publication state
Published
Issued date
08/1992
Volume
32
Number
4
Pages
369-77
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Research Support, Non-U.S. Gov't --- Old month value: Aug
Abstract
Human autoantibodies were used to localize centromere proteins by immunoelectron microscopy, immunofluorescence, and confocal microscopy in isolated cells and in cryosections of rabbit testis. A computer-assisted three-dimensional reconstruction of the positions and sizes of fluorescent spots allowed us to follow their movements during the different phases of spermiogenesis. In very young spermatids, the centromeres were distributed within a space separated from both the external nuclear limits and the nuclear core. They moved towards the nuclear center in cap phase spermatids, where they clustered into a few large centromeric masses. In preelongating spermatids, the immunolabeled proteins were dispersed within an equatorial area, where they formed one large mass. In late spermatids, the mass moved towards the posterior part of the nucleus, and, in the spermatozoon, the two basal knobs located at the base of the nuclei were the only strongly immunolabeled structures, while no labeling of the main part of the nucleus was observed. Since the number of centromeres remained close to the number of chromosomes until the cap phase of spermatid differentiation, we hypothesize that the labeling of young spermatids corresponds to centrometric proteins associated with their specific DNA counterparts, while the centromere proteins, possibly detached from their DNA loci, were released from nuclei of old spermatids in the same way as are histones and transition proteins.
Keywords
Animals
*Autoantigens
Centromere/*metabolism/ultrastructure
Centromere Protein B
Chromosomal Proteins, Non-Histone/*metabolism
DNA/metabolism
DNA-Binding Proteins/metabolism
Fluorescent Antibody Technique
Humans
Male
Rabbits
Spermatids/cytology/*metabolism/ultrastructure
Spermatogenesis
Pubmed
Web of science
Create date
25/01/2008 13:56
Last modification date
20/08/2019 14:03