A serum metabolomics signature of hypothermia fatalities involving arginase activity, tryptophan content, and phosphatidylcholine saturation.

Details

Serval ID
serval:BIB_24C9FBC1BF17
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A serum metabolomics signature of hypothermia fatalities involving arginase activity, tryptophan content, and phosphatidylcholine saturation.
Journal
International journal of legal medicine
Author(s)
Rousseau G., Chao de la Barca J.M., Rougé-Maillart C., Teresiński G., Jousset N., Dieu X., Chabrun F., Prunier-Mirabeau D., Simard G., Reynier P., Palmiere C.
ISSN
1437-1596 (Electronic)
ISSN-L
0937-9827
Publication state
Published
Issued date
05/2019
Peer-reviewed
Oui
Volume
133
Number
3
Pages
889-898
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Hypothermia is a potentially lethal condition whose postmortem diagnosis is often complex to perform due to the absence of pathognomonic lesions and biomarkers. Our first study of human serum and urinary metabolome in hypothermia fatalities sought novel biomarkers with better diagnostic performances than those already existing.
Thirty-two cases of hypothermia deaths and 16 cases excluding known antemortem exposure to cold or postmortem elements suggesting hypothermia were selected. A targeted metabolomic study allowing the detection and quantitation of 188 metabolites was performed on collected serum and urine using direct flow injection (FIA) and liquid chromatography (LC) separation, both coupled to tandem mass spectrometry (MS/MS). Amino acid quantification was also carried on using an in-house LC-MS/MS method in order to replicate the results obtained with the metabolomic study.
A discriminant metabolic signature allowing a clear separation between hypothermia and control groups was obtained in the serum. This signature was characterized by increased arginase activity and fatty acid unsaturation along with decreased levels of tryptophan in hypothermia fatalities compared to controls. By contrast, no discriminant metabolic signature separating hypothermia from control fatalities was found in urines.
The serum metabolic signature of hypothermia fatalities herein observed pointed toward metabolic adaptations that likely aimed at heat production enhancement, endothelial function, and cell membrane fluidity preservation. Novel biomarkers potentially useful in a hypothermia diagnosis were also identified.
Keywords
Adult, Aged, Aged, 80 and over, Arginase/metabolism, Biomarkers/metabolism, Case-Control Studies, Chromatography, Liquid, Female, Humans, Hypothermia/metabolism, Male, Metabolomics, Middle Aged, Phosphatidylcholines/metabolism, Tandem Mass Spectrometry, Tryptophan/metabolism, Biomarkers, Forensic science, Hypothermia, Serum, Urine
Pubmed
Web of science
Create date
20/05/2019 16:26
Last modification date
20/08/2019 13:03
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