Determination of the novel non-peptidic HIV-protease inhibitor tipranavir by HPLC-UV after solid-phase extraction.

Details

Serval ID
serval:BIB_240D221E7E2F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Determination of the novel non-peptidic HIV-protease inhibitor tipranavir by HPLC-UV after solid-phase extraction.
Journal
Journal of Chromatography. B
Author(s)
Colombo S., Béguin A., Marzolini C., Telenti A., Biollaz J., Decosterd L.A.
ISSN
1570-0232
Publication state
Published
Issued date
02/2006
Peer-reviewed
Oui
Volume
832
Number
1
Pages
138-143
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
An HPLC method previously described for the assay of amprenavir (APV), ritonavir (RTV), indinavir (IDV), saquinavir (SQV), nelfinavir (NFV), lopinavir (LPV), atazanavir (ATV), nevirapine (NVP) and efavirenz (EFV) can be also conveniently applied, with minor gradient program adjustment, for the determination of the novel non-peptidic HIV protease inhibitor tipranavir (TPV) in human plasma, by off-line solid-phase extraction (SPE) followed by HPLC coupled with UV-diode array detection (DAD). After viral inactivation by heat, the plasma is diluted with phosphate buffer (pH 7), and subjected to a SPE on a C18 cartridge. Matrix components are eliminated with a solution of 0.1% H3PO4 solution neutralised to pH 7, and TPV is eluted with MeOH. The resulting eluate is evaporated and reconstituted in 100 microl MeOH/H2O 50/50. A 40 microl volume is injected onto a Nucleosil C18 AB column and TPV is analysed by UV detection at 201 nm using a gradient elution program constituted of MeCN and phosphate buffer adjusted to pH 5.12 and containing 0.02% sodium heptanesulfonate. The calibration curves are linear up to 75 microg/ml, with a lower limit of quantification of 0.125 microg/ml. The mean absolute recovery of TPV is 77.1+/-4.0%. The method is precise with mean inter-day coefficient of variations (CVs) within 2.2-3.4%, and accurate (range of inter-day deviations from 0.7 to 1.2%). The method has been validated and is currently applied to the monitoring of TPV plasma levels in HIV patients.
Keywords
Calibration, Chromatography, High Pressure Liquid/methods, HIV Protease Inhibitors/blood, Humans, Pyridines/blood, Pyrones/blood, Reference Standards, Reproducibility of Results, Sensitivity and Specificity, Spectrophotometry, Ultraviolet/methods
Pubmed
Web of science
Create date
25/01/2008 11:41
Last modification date
20/08/2019 14:01
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