Sawhorse-type diruthenium tetracarbonyl complexes containing porphyrin-derived ligands as highly selective photosensitizers for female reproductive cancer cells.

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Serval ID
serval:BIB_238114A205C4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Sawhorse-type diruthenium tetracarbonyl complexes containing porphyrin-derived ligands as highly selective photosensitizers for female reproductive cancer cells.
Journal
Journal of Biological Inorganic Chemistry
Author(s)
Schmitt F., Auzias M., Stepnicka P., Sei Y., Yamaguchi K., Süss-Fink G., Therrien B., Juillerat-Jeanneret L.
ISSN
1432-1327[electronic]
Publication state
Published
Issued date
2009
Volume
14
Number
5
Pages
693-701
Language
english
Abstract
Diruthenium tetracarbonyl complexes of the type [Ru2(CO)4(l2-g2-O2CR)2L2] containing a Ru-Ru backbone with four equatorial carbonyl ligands, two carboxylato bridges, and two axial two-electron ligands in a sawhorse-like geometry have been synthesized with porphyrin-derived substituents in the axial ligands [1: R is CH3, L is 5-(4-pyridyl)-10,15,20-triphenyl-21,23H-porphyrin], in the bridging carboxylato ligands [2: RCO2H is 5-(4-carboxyphenyl)-10,15,20-triphenyl-21,23H-porphyrin, L is PPh3; 3: RCO2H is 5-(4-carboxyphenyl)-10,15,20-triphenyl-21,23H-porphyrin, L is 1,3,5-triaza-7-phosphatricyclo [3.3.1.1]decane], or in both positions [4: RCO2H is 5-(4-carboxyphenyl)-10,15,20-triphenyl-21,23H-porphyrin, L is 5-(4-pyridyl)-10,15,20-triphenyl-21,23H-porphyrin]. Compounds 1-3 were assessed on different types of human cancer cells and normal cells. Their uptake by cells was quantified by fluorescence and checked by fluorescence microscopy. These compounds were taken up by human HeLa cervix and A2780 and Ovcar ovarian carcinoma cells but not by normal cells and other cancer cell lines (A549 pulmonary, Me300 melanoma, PC3 and LnCap prostate, KB head and neck, MDAMB231 and MCF7 breast, or HT29 colon cancer cells). The compounds demonstrated no cytotoxicity in the absence of laser irradiation but exhibited good phototoxicities in HeLa and A2780 cells when exposed to laser light at 652 nm, displaying an LD50 between 1.5 and 6.5 J/cm2 in these two cell lines and more than 15 J/cm2 for the others. Thus, these types of porphyric compound present specificity for cancer cell lines of the female reproductive system and not for normal cells; thus being promising new organometallic photosensitizers.
Keywords
Antineoplastic Agents/chemical synthesis, Antineoplastic Agents/chemistry, Carcinoma/drug therapy, Cell Line, Tumor, Cell Survival/drug effects, Cells, Cultured, Electrochemistry, Female, Fibroblasts/cytology, Fibroblasts/drug effects, Humans, Molecular Structure, Organometallic Compounds/chemical synthesis, Organometallic Compounds/chemistry, Ovarian Neoplasms/drug therapy, Photosensitizing Agents/chemical synthesis, Photosensitizing Agents/chemistry, Porphyrins/chemical synthesis, Porphyrins/chemistry, Spectrum Analysis, Uterine Cervical Neoplasms/drug therapy
Pubmed
Web of science
Open Access
Yes
Create date
20/05/2009 18:24
Last modification date
01/10/2019 6:17
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