A prevalent and culturable microbiota links ecological balance to clinical stability of the human lung after transplantation.

Details

Serval ID
serval:BIB_231E626287AA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A prevalent and culturable microbiota links ecological balance to clinical stability of the human lung after transplantation.
Journal
Nature communications
Author(s)
Das S., Bernasconi E., Koutsokera A., Wurlod D.A., Tripathi V., Bonilla-Rosso G., Aubert J.D., Derkenne M.F., Mercier L., Pattaroni C., Rapin A., von Garnier C., Marsland B.J., Engel P., Nicod L.P.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
09/04/2021
Peer-reviewed
Oui
Volume
12
Number
1
Pages
2126
Language
english
Notes
Publication types: Journal Article ; Observational Study ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
There is accumulating evidence that the lower airway microbiota impacts lung health. However, the link between microbial community composition and lung homeostasis remains elusive. We combine amplicon sequencing and bacterial culturing to characterize the viable bacterial community in 234 longitudinal bronchoalveolar lavage samples from 64 lung transplant recipients and establish links to viral loads, host gene expression, lung function, and transplant health. We find that the lung microbiota post-transplant can be categorized into four distinct compositional states, 'pneumotypes'. The predominant 'balanced' pneumotype is characterized by a diverse bacterial community with moderate viral loads, and host gene expression profiles suggesting immune tolerance. The other three pneumotypes are characterized by being either microbiota-depleted, or dominated by potential pathogens, and are linked to increased immune activity, lower respiratory function, and increased risks of infection and rejection. Collectively, our findings establish a link between the lung microbial ecosystem, human lung function, and clinical stability post-transplant.
Keywords
Adult, Allografts/immunology, Allografts/microbiology, Bacteria/genetics, Bacteria/immunology, Bacteria/isolation & purification, Bacteria/pathogenicity, Bacterial Load/immunology, Bacteriological Techniques, Bronchoalveolar Lavage Fluid/microbiology, Bronchoscopy, DNA, Bacterial/isolation & purification, Female, Graft Rejection/diagnosis, Graft Rejection/immunology, Graft Rejection/microbiology, Humans, Immune Tolerance, Longitudinal Studies, Lung/immunology, Lung/microbiology, Lung Transplantation/adverse effects, Male, Metagenomics, Microbiota/genetics, Microbiota/immunology, Middle Aged, Pneumonia, Bacterial/diagnosis, Pneumonia, Bacterial/immunology, Pneumonia, Bacterial/microbiology, Prospective Studies, RNA, Ribosomal, 16S/genetics
Pubmed
Web of science
Open Access
Yes
Create date
10/04/2021 19:33
Last modification date
04/09/2021 6:34
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