Stable mRNP formation and export require cotranscriptional recruitment of the mRNA export factors Yra1p and Sub2p by Hpr1p.

Details

Serval ID
serval:BIB_23049
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Stable mRNP formation and export require cotranscriptional recruitment of the mRNA export factors Yra1p and Sub2p by Hpr1p.
Journal
Molecular and cellular biology
Author(s)
Zenklusen D., Vinciguerra P., Wyss J.C., Stutz F.
ISSN
0270-7306
Publication state
Published
Issued date
2002
Volume
22
Number
23
Pages
8241-8253
Language
english
Notes
Publication types: Journal Article
Abstract
Yra1p/REF participates in mRNA export by recruiting the export receptor Mex67p to messenger ribonucleoprotein (mRNP) complexes. Yra1p also binds Sub2p, a DEAD box ATPase/RNA helicase implicated in splicing and required for mRNA export. We identified genetic and physical interactions between Yra1p, Sub2p, and Hpr1p, a protein involved in transcription elongation whose deletion leads to poly(A)(+) RNA accumulation in the nucleus. By chromatin immunoprecipitation (ChIP) experiments, we show that Hpr1p, Sub2p, and Yra1p become associated with active genes during transcription elongation and that Hpr1p is required for the efficient recruitment of Sub2p and Yra1p. The data indicate that transcription and export are functionally linked and that mRNA export defects may be due in part to inefficient loading of essential mRNA export factors on the growing mRNP. We also identified functional interactions between Yra1p and the exosome components Rrp45p and Rrp6p. We show that yra1, sub2, and Deltahpr1 mutants all present defects in mRNA accumulation and that deletion of RRP6 in yra1 mutants restores normal mRNA levels. The data support the hypothesis that an exosome-dependent surveillance mechanism targets improperly assembled mRNPs for degradation.
Keywords
Active Transport, Cell Nucleus/physiology, Adenosine Triphosphatases/metabolism, DNA-Binding Proteins/metabolism, Genes, Reporter, Mutation, Nuclear Proteins/metabolism, Protein Binding, Proton-Translocating ATPases/genetics, Proton-Translocating ATPases/metabolism, RNA Helicases/metabolism, RNA, Messenger/metabolism, RNA-Binding Proteins/metabolism, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/metabolism, Ribonucleoproteins/metabolism, Saccharomyces cerevisiae/physiology, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism, Transcription, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 13:18
Last modification date
20/08/2019 14:00
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