Modulation of Shoot Phosphate Level and Growth by PHOSPHATE1 Upstream Open Reading Frame.

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Serval ID
serval:BIB_22AE09E8C47E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Modulation of Shoot Phosphate Level and Growth by PHOSPHATE1 Upstream Open Reading Frame.
Journal
Plant physiology
Author(s)
Reis R.S., Deforges J., Sokoloff T., Poirier Y.
ISSN
1532-2548 (Electronic)
ISSN-L
0032-0889
Publication state
Published
Issued date
07/2020
Peer-reviewed
Oui
Volume
183
Number
3
Pages
1145-1156
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Inorganic orthophosphate (Pi) is an essential nutrient for plant growth, and its availability strongly impacts crop yield. PHOSPHATE1 (PHO1) transfers Pi from root to shoot via Pi export into root xylem vessels. In this work, we demonstrate that an upstream open reading frame (uORF) present in the 5' untranslated region of the Arabidopsis (Arabidopsis thaliana) PHO1 inhibits its translation and influences Pi homeostasis. The presence of the uORF strongly inhibited the translation of a PHO1 5'UTR-luciferase construct in protoplasts. A point mutation removing the PHO1 uORF (ΔuORF) in transgenic Arabidopsis resulted in increased association of its mRNA with polysomes and led to higher PHO1 protein levels, independent of Pi availability. Interestingly, deletion of the uORF led to higher shoot Pi content and was associated with improved shoot growth under low external Pi supply and no deleterious effects under Pi-sufficient conditions. We further show that natural accessions lacking the PHO1 uORF exhibit higher PHO1 protein levels and shoot Pi content. Increased shoot Pi content was linked to the absence of the PHO1 uORF in a population of F2 segregants. We identified the PHO1 uORF in genomes of crops such as rice (Oryza sativa), maize (Zea mays), barley (Hordeum vulgare), and wheat (Triticum aesativum), and we verified the inhibitory effect of the rice PHO1 uORF on translation in protoplasts. Our work suggests that regulation of PHO1 expression via its uORF might be a genetic resource useful-both in natural populations and in the context of genome editing-toward improving plant growth under Pi-deficient conditions.
Keywords
Arabidopsis/genetics, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Base Sequence, Ecotype, Gene Deletion, Gene Expression Regulation, Plant, Inheritance Patterns/genetics, Open Reading Frames/genetics, Phosphates/deficiency, Phosphates/metabolism, Plant Shoots/metabolism, Protein Biosynthesis, RNA, Messenger/genetics, RNA, Messenger/metabolism
Pubmed
Web of science
Open Access
Yes
Funding(s)
Swiss National Science Foundation / CRSII3_154471
Swiss National Science Foundation / CRSII3_154471
Create date
30/09/2020 13:50
Last modification date
25/04/2021 6:34
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