Direct transfer of antigen-specific cytolytic activity to noncytolytic cells upon fusion with liposomes derived from cytolytic T cell clones

Details

Serval ID
serval:BIB_2298B5B3A805
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Direct transfer of antigen-specific cytolytic activity to noncytolytic cells upon fusion with liposomes derived from cytolytic T cell clones
Journal
Journal of Experimental Medicine
Author(s)
Harris  D. T., MacDonald  H. R., Cerottini  J. C.
ISSN
0022-1007 (Print)
Publication state
Published
Issued date
01/1984
Volume
159
Number
1
Pages
261-75
Notes
Journal Article --- Old month value: Jan 1
Abstract
Murine cytolytic T lymphocytes (CTL) clones were solubilized in Nonidet P-40 detergent, and the matrix and membrane proteins separated from the nuclear constituents. These proteins, in combination with exogenous lipids and Sendai virus envelope proteins, were used to construct liposomes that were then fused with noncytolytic cloned T cell recipients. The resultant fusion products were found to be highly cytolytic and appeared to express the same specificity as the original donor clone. Further analysis showed that the liposomal transfer process was extremely efficient. Moreover, in addition to noncytolytic T cell clones, three transformed T cell lines and one B cell line were found to express specific cytolytic activity after fusion with appropriate liposomes. Inhibition experiments using monoclonal antibodies against target cell antigens, as well as analysis of the lytic reactivity pattern of the fusion products, confirmed the high degree of specificity conferred to the recipient cells. This study thus indicates that the two characteristics typically associated with CTL, namely antigen-specific recognition and cytolytic activity, can be solubilized from CTL and transferred to recipient cells that do not express these characteristics.
Keywords
Animals B-Lymphocytes/immunology Cell Line Clone Cells/immunology *Cytotoxicity, Immunologic Dose-Response Relationship, Immunologic *Epitopes H-2 Antigens/immunology Liposomes/administration & dosage/*immunology Lymphocyte Activation Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred DBA Subcellular Fractions/immunology T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:14
Last modification date
20/08/2019 13:00
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