Cell-Free Assay for Inflammasome Activation.

Details

Serval ID
serval:BIB_2191EA9C70BB
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Title
Cell-Free Assay for Inflammasome Activation.
Journal
Methods in molecular biology
Author(s)
Jamilloux Y., Martinon F.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
1417
Pages
207-215
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Inflammasomes are multiprotein complexes, which assembly results in caspase-1 activation and subsequent IL-1β and IL-18 activation and secretion. In a cell-free system, based on cytosols of normally growing cells, the disruption of the cell membrane spontaneously activates the inflammasome. Studying the activation of the inflammasome in cytosolic extracts provides multiple advantages, as it is synchronized, rapid, strong, and mostly plasma membrane-free. This protocol covers the methods required to prepare cell lysates and study inflammasome activation using different read-outs. General considerations are provided that may help in the design of modified methods. This assay can be useful to study potential inflammasome interactors and the signaling pathways involved in its activation.

Keywords
Cell-Free System/metabolism, Cytosol/metabolism, Humans, Inflammasomes/metabolism, THP-1 Cells, ASC, Cell-free assay, IL-1β, Inflammasome, NLRP3
Pubmed
Create date
26/05/2016 13:46
Last modification date
20/08/2019 13:58
Usage data