Cytolytic T lymphocyte recognition of the immunodominant HLA-A*0201-restricted Melan-A/MART-1 antigenic peptide in melanoma

Details

Serval ID
serval:BIB_2140E37E267C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cytolytic T lymphocyte recognition of the immunodominant HLA-A*0201-restricted Melan-A/MART-1 antigenic peptide in melanoma
Journal
Journal of Immunology
Author(s)
Romero  P., Gervois  N., Schneider  J., Escobar  P., Valmori  D., Pannetier  C., Steinle  A., Wolfel  T., Lienard  D., Brichard  V., van Pel  A., Jotereau  F., Cerottini  J. C.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
09/1997
Volume
159
Number
5
Pages
2366-74
Notes
Comparative Study
Journal Article --- Old month value: Sep 1
Abstract
The Melan-A/MART-1 gene product is frequently recognized by tumor-specific HLA-A2-restricted CTL. An immunodominant nonapeptide has been localized to the region spanning residues 27-35. However, the decapeptide including residues 26-35 (the nonapeptide extended NH2 terminally by one residue) appeared to be recognized as efficiently as the nonapeptide. In this study, we show that the optimal length immunodominant peptide appears to correspond to the decapeptide 26-35, as assessed by quantitative analyses of both 4 polyclonal and 13 monoclonal populations of specific CTL. Functional assays of peptide binding to HLA-A2 indicate that the decapeptide is significantly a more efficient binder than the nonapeptide. Moreover, analogues of the decapeptide including substitutions at a secondary HLA-A2 peptide anchor further improve decapeptide binding. Finally, we show that the functional (9 CTL clones analyzed) and structural TCR repertoire (7 CTL clones) of a group of specific CTL clones is rather diverse. The findings reported here may have important implications for future peptide-based melanoma vaccination trials as well as for the monitoring of specific CTL responses in vivo.
Keywords
Amino Acid Sequence Antigen Presentation Antigens, Neoplasm/genetics/*immunology Base Sequence Clone Cells/immunology Gene Rearrangement, T-Lymphocyte HLA-A2 Antigen/*immunology Humans Immunodominant Epitopes/genetics/*immunology Lymphocytes, Tumor-Infiltrating/*immunology Melanoma/*immunology Molecular Sequence Data Neoplasm Proteins/genetics/*immunology Point Mutation Protein Binding Receptors, Antigen, T-Cell, alpha-beta/genetics Substrate Specificity T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Create date
28/01/2008 11:13
Last modification date
20/08/2019 12:57
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