Human keratinocytes produce the complement inhibitor factor I: Synthesis is regulated by interferon-gamma.

Details

Serval ID
serval:BIB_213509B61F91
Type
Article: article from journal or magazin.
Collection
Publications
Title
Human keratinocytes produce the complement inhibitor factor I: Synthesis is regulated by interferon-gamma.
Journal
Molecular immunology
Author(s)
Timár K.K., Junnikkala S., Dallos A., Jarva H., Bhuiyan Z.A., Meri S., Bos J.D., Asghar S.S.
ISSN
0161-5890 (Print)
ISSN-L
0161-5890
Publication state
Published
Issued date
04/2007
Peer-reviewed
Oui
Volume
44
Number
11
Pages
2943-2949
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Extrahepatic complement synthesis is believed to play an important role in host defense and inflammation at tissue and organ level. In the epidermis the most abundant cell type, keratinocytes have been shown to produce C3, factor B and factor H. In the present study, we investigated the synthesis of factor I by human keratinocytes. We also studied whether proinflammatory cytokines IL-1alpha, IL-6, TGF-beta1, TNF-alpha and IFN-gamma regulate factor I synthesis in keratinocytes. Human keratinocytes constitutively expressed factor I mRNA and produced factor I protein. Amongst the above-mentioned cytokines, only IFN-gamma regulated the synthesis of factor I, and this effect occurred predominantly at pre-translational level. Factor I produced by keratinocytes was functionally active in cleaving C3b. In conclusion, we demonstrate that keratinocytes are capable of synthesizing factor I, and that this synthesis is regulated by IFN-gamma.

Keywords
Cells, Cultured, Complement Factor I/biosynthesis, Complement Factor I/genetics, Cytokines/pharmacology, Gene Expression Regulation/drug effects, Humans, Interferon-gamma/pharmacology, Keratinocytes/metabolism, Protein Biosynthesis/drug effects, RNA, Messenger/biosynthesis, RNA, Messenger/genetics, Recombinant Proteins
Pubmed
Web of science
Create date
01/03/2018 15:35
Last modification date
20/08/2019 12:57
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