Brain spectrin(240/235A): a novel astrocyte specific spectrin isoform.

Details

Serval ID
serval:BIB_210A4CD9C016
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Brain spectrin(240/235A): a novel astrocyte specific spectrin isoform.
Journal
Brain Research Bulletin
Author(s)
Goodman S.R., Lopresti L.L., Riederer B.M., Sikorski A., Zagon I.S.
ISSN
0361-9230 (Print)
ISSN-L
0361-9230
Publication state
Published
Issued date
1989
Volume
23
Number
4-5
Pages
311-316
Language
english
Abstract
We have previously demonstrated the existence of two distinct isoforms of spectrin in mammalian brain (23). Brain spectrin(240/235) is found primarily in neuronal axons and presynaptic terminals, and brain spectrin(240/235E) is located in neuronal cell bodies, dendrites and postsynaptic terminals, and oligodendrocytes. These isoforms are thought to play important roles in controlling the early events of synaptic transmission, axonal transport of organelles and vesicles, and lateral mobility of integral membrane proteins. In this study, we have utilized a panel of monoclonal antibodies to identify a novel astrocyte specific isoform(240/235A) with subunits of 240 kDa and 235 kDa in a 1:1 ratio. Double label indirect immunofluorescence has indicated that brain spectrin (240/235A) is distinct from brain spectrin (240/235E). This novel isoform located in the soma and processes of astrocytes may play a role in actin-membrane attachment, cellular architecture, strengthening of the membrane fabric, and translocation of cytoplasmic organelles and vesicles.
Keywords
Animals, Antibodies, Monoclonal/diagnostic use, Astrocytes/metabolism, Brain/metabolism, Immunohistochemistry, Molecular Weight, Rats, Spectrin/immunology
Pubmed
Web of science
Create date
24/01/2008 14:34
Last modification date
20/08/2019 12:57
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