Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand.

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Version: Final published version
License: CC BY-NC-SA 4.0
Serval ID
serval:BIB_20FF0D9FD4AB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand.
Journal
The Journal of experimental medicine
Author(s)
Renno T., Hahne M., Tschopp J., MacDonald H.R.
ISSN
0022-1007
Publication state
Published
Issued date
1996
Peer-reviewed
Oui
Volume
183
Number
2
Pages
431-437
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Staphylococcal enterotoxin B (SEB) is a bacterial superantigen (SAg) that predominantly interacts with V(beta)8+ T cells. In vivo treatment of mice with SEB leads to an initial increase in the percentage of V(beta)8+ T cells, followed by a decrease in the numbers of these cells, eventually reaching lower levels than those found before treatment with the SAg. This decrease is due to apoptosis of the SEB-responding cells. In the present study, we use the distinct light scattering characteristics of apoptotic cells to characterize T cells that are being deleted in response to SEB in vivo. We show that dying, SEB-reactive T cells express high levels of Fas and Fas ligand (Fas-L), which are implicated in apoptotic cell death. In addition, the B cell marker B220 is upregulated on apoptotic cells. Moreover, we show that the generation of cells with an apoptotic phenotype is severely impaired in response to SEB in functional Fas-L-deficient mutant gld mice, confirming the role of the Fas pathway in SAg mediated peripheral deletion in vivo.
Keywords
Animals, Antigens, CD45/biosynthesis, Antigens, CD95/biosynthesis, Apoptosis, B-Lymphocytes/immunology, Enterotoxins/immunology, Epitopes, Fas Ligand Protein, Flow Cytometry, Gene Expression Regulation, Developmental, Membrane Glycoproteins/biosynthesis, Mice, Mice, Inbred BALB C, Receptors, Antigen, T-Cell, alpha-beta/biosynthesis, Scattering, Radiation, Superantigens/immunology, T-Lymphocyte Subsets/immunology, T-Lymphocytes/immunology, Up-Regulation
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:18
Last modification date
20/08/2019 12:57
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