Size and Content of the Sex-Determining Region of the Y Chromosome in Dioecious <i>Mercurialis annua</i>, a Plant with Homomorphic Sex Chromosomes.

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State: Public
Version: Final published version
Serval ID
serval:BIB_20750E6695BD
Type
Article: article from journal or magazin.
Collection
Publications
Title
Size and Content of the Sex-Determining Region of the Y Chromosome in Dioecious <i>Mercurialis annua</i>, a Plant with Homomorphic Sex Chromosomes.
Journal
Genes
Author(s)
Veltsos P., Cossard G., Beaudoing E., Beydon G., Savova Bianchi D., Roux C., C González-Martínez S., R Pannell J.
ISSN
2073-4425 (Print)
ISSN-L
2073-4425
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
9
Number
6
Pages
NA
Language
english
Abstract
Dioecious plants vary in whether their sex chromosomes are heteromorphic or homomorphic, but even homomorphic sex chromosomes may show divergence between homologues in the non-recombining, sex-determining region (SDR). Very little is known about the SDR of these species, which might represent particularly early stages of sex-chromosome evolution. Here, we assess the size and content of the SDR of the diploid dioecious herb <i>Mercurialis annua</i> , a species with homomorphic sex chromosomes and mild Y-chromosome degeneration. We used RNA sequencing (RNAseq) to identify new Y-linked markers for <i>M. annua.</i> Twelve of 24 transcripts showing male-specific expression in a previous experiment could be amplified by polymerase chain reaction (PCR) only from males, and are thus likely to be Y-linked. Analysis of genome-capture data from multiple populations of <i>M. annua</i> pointed to an additional six male-limited (and thus Y-linked) sequences. We used these markers to identify and sequence 17 sex-linked bacterial artificial chromosomes (BACs), which form 11 groups of non-overlapping sequences, covering a total sequence length of about 1.5 Mb. Content analysis of this region suggests that it is enriched for repeats, has low gene density, and contains few candidate sex-determining genes. The BACs map to a subset of the sex-linked region of the genetic map, which we estimate to be at least 14.5 Mb. This is substantially larger than estimates for other dioecious plants with homomorphic sex chromosomes, both in absolute terms and relative to their genome sizes. Our data provide a rare, high-resolution view of the homomorphic Y chromosome of a dioecious plant.
Keywords
RNA sequencing (RNAseq), bacterial artificial chromosomes (BAC), gene density, genetic map, transposable element
Pubmed
Web of science
Open Access
Yes
Create date
31/05/2018 16:48
Last modification date
20/08/2019 12:56
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