Insights into Mobile Genetic Elements of the Biocide-Degrading Bacterium Pseudomonas nitroreducens HBP-1.

Details

Serval ID
serval:BIB_206C29759190
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Insights into Mobile Genetic Elements of the Biocide-Degrading Bacterium Pseudomonas nitroreducens HBP-1.
Journal
Genes
Author(s)
Carraro N., Sentchilo V., Polák L., Bertelli C., van der Meer J.R.
ISSN
2073-4425 (Electronic)
ISSN-L
2073-4425
Publication state
Published
Issued date
12/08/2020
Peer-reviewed
Oui
Volume
11
Number
8
Pages
930
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
The sewage sludge isolate Pseudomonas nitroreducens HBP-1 was the first bacterium known to completely degrade the fungicide 2-hydroxybiphenyl. PacBio and Illumina whole-genome sequencing revealed three circular DNA replicons: a chromosome and two plasmids. Plasmids were shown to code for putative adaptive functions such as heavy metal resistance, but with unclarified ability for self-transfer. About one-tenth of strain HBP-1's chromosomal genes are likely of recent horizontal influx, being part of genomic islands, prophages and integrative and conjugative elements (ICEs). P. nitroreducens carries two large ICEs with different functional specialization, but with homologous core structures to the well-known ICEclc of Pseudomonas knackmussii B13. The variable regions of ICEPni1 (96 kb) code for, among others, heavy metal resistances and formaldehyde detoxification, whereas those of ICEPni2 (171 kb) encodes complete meta-cleavage pathways for catabolism of 2-hydroxybiphenyl and salicylate, a protocatechuate pathway and peripheral enzymes for 4-hydroxybenzoate, ferulate, vanillin and vanillate transformation. Both ICEs transferred at frequencies of 10 <sup>-6</sup> -10 <sup>-8</sup> per P. nitroreducens HBP-1 donor into Pseudomonas putida, where they integrated site specifically into tRNA <sup>Gly</sup> -gene targets, as expected. Our study highlights the underlying determinants and mechanisms driving dissemination of adaptive properties allowing bacterial strains to cope with polluted environments.
Keywords
ICEclc, Pseudomonas azelaica, adaptation, aromatic compounds, evolution, heavy metal, integrative and conjugative elements
Pubmed
Web of science
Open Access
Yes
Create date
28/08/2020 7:47
Last modification date
20/01/2021 6:26
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