MAP kinase pathways in UV-induced apoptosis of retinal pigment epithelium ARPE19 cells.

Details

Ressource 1Download: serval:BIB_1FF6934DDA79.P001 (508.05 [Ko])
State: Public
Version: author
License: Not specified
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
Serval ID
serval:BIB_1FF6934DDA79
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
MAP kinase pathways in UV-induced apoptosis of retinal pigment epithelium ARPE19 cells.
Journal
Apoptosis
Author(s)
Roduit R., Schorderet D.F.
ISSN
1573-675X[electronic]
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
13
Number
3
Pages
343-353
Language
english
Notes
Publication types: Journal Article - Publication Status: ppublish
Abstract
The retinal pigment epithelium (RPE) is constantly exposed to external injuries which lead to degeneration, dysfunction or loss of RPE cells. The balance between RPE cells death and proliferation may be responsible for several diseases of the underlying retina, including age-related macular degeneration (AMD) and proliferative vitreoretinopathy (PVR). Signaling pathways able to control cells proliferation or death usually involve the MAPK (mitogen-activated protein kinases) pathways, which modulate the activity of transcription factors by phosphorylation. UV exposure induces DNA breakdown and causes cellular damage through the production of reactive oxygen species (ROS) leading to programmed cell death. In this study, human retinal pigment epithelial cells ARPE19 were exposed to 100 J/m(2) of UV-C and MAPK pathways were studied. We first showed the expression of the three major MAPK pathways. Then we showed that activator protein-1 (AP-1) was activated through phosphorylation of cJun and cFos, induced by JNK and p38, respectively. Specific inhibitors of both kinases decreased their respective activities and phosphorylation of their nuclear targets (cJun and cFos) and reduced UV-induced cell death. The use of specific kinases inhibitors may provide excellent tools to prevent RPE apoptosis specifically in RPE diseases involving ROS and other stress-related compounds such as in AMD.
Keywords
Apoptosis/physiology, Apoptosis/radiation effects, Cell Line, Humans, MAP Kinase Kinase 4/metabolism, MAP Kinase Signaling System/physiology, Mitogen-Activated Protein Kinase 1/metabolism, Mitogen-Activated Protein Kinase 8/antagonists & inhibitors, Mitogen-Activated Protein Kinase 8/metabolism, Mitogen-Activated Protein Kinase 9/antagonists & inhibitors, Mitogen-Activated Protein Kinase 9/metabolism, Pigment Epithelium of Eye/cytology, Pigment Epithelium of Eye/physiopathology, Proto-Oncogene Proteins c-fos/metabolism, Retina/enzymology, Retina/radiation effects, Transcription Factor AP-1/metabolism, Ultraviolet Rays, p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors, p38 Mitogen-Activated Protein Kinases/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
05/10/2009 16:35
Last modification date
01/10/2019 7:17
Usage data