Lymph node migratory dendritic cells modulate HIV-1 transcription through PD-1 engagement.

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State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_1FF4BBBC8958
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Lymph node migratory dendritic cells modulate HIV-1 transcription through PD-1 engagement.
Journal
PLoS pathogens
Author(s)
Banga R., Rebecchini C., Procopio F.A., Noto A., Munoz O., Ioannidou K., Fenwick C., Ohmiti K., Cavassini M., Corpataux J.M., de Leval L., Pantaleo G., Perreau M.
ISSN
1553-7374 (Electronic)
ISSN-L
1553-7366
Publication state
Published
Issued date
07/2019
Peer-reviewed
Oui
Volume
15
Number
7
Pages
e1007918
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
T-follicular helper (Tfh) cells, co-expressing PD-1 and TIGIT, serve as a major cell reservoir for HIV-1 and are responsible for active and persistent HIV-1 transcription after prolonged antiretroviral therapy (ART). However, the precise mechanisms regulating HIV-1 transcription in lymph nodes (LNs) remain unclear. In the present study, we investigated the potential role of immune checkpoint (IC)/IC-Ligand (IC-L) interactions on HIV-1 transcription in LN-microenvironment. We show that PD-L1 (PD-1-ligand) and CD155 (TIGIT-ligand) are predominantly co-expressed on LN migratory (CD1chighCCR7+CD127+) dendritic cells (DCs), that locate predominantly in extra-follicular areas in ART treated individuals. We demonstrate that TCR-mediated HIV production is suppressed in vitro in the presence of recombinant PD-L1 or CD155 and, more importantly, when LN migratory DCs are co-cultured with PD-1+/Tfh cells. These results indicate that LN migratory DCs expressing IC-Ls may more efficiently restrict HIV-1 transcription in the extra-follicular areas and explain the persistence of HIV transcription in PD-1+/Tfh cells after prolonged ART within germinal centers.
Keywords
Anti-HIV Agents/therapeutic use, Antibodies, Monoclonal, Humanized/administration & dosage, Cell Movement/immunology, Cellular Microenvironment/immunology, Coculture Techniques, Dendritic Cells/immunology, Dendritic Cells/virology, Germinal Center/immunology, Germinal Center/virology, HIV Infections/drug therapy, HIV Infections/immunology, HIV Infections/virology, HIV-1/genetics, HIV-1/immunology, HIV-1/pathogenicity, Host Microbial Interactions/immunology, Humans, In Vitro Techniques, Lymph Nodes/immunology, Lymph Nodes/virology, Programmed Cell Death 1 Ligand 2 Protein/metabolism, Programmed Cell Death 1 Receptor/antagonists & inhibitors, Programmed Cell Death 1 Receptor/metabolism, Receptors, Immunologic/metabolism, Receptors, Virus/metabolism, T-Lymphocytes, Helper-Inducer/immunology, T-Lymphocytes, Helper-Inducer/virology, Transcription, Genetic, Virulence
Pubmed
Web of science
Open Access
Yes
Create date
23/07/2019 12:46
Last modification date
11/01/2020 7:16
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