Effect of changes in the deuterium content of drinking water on the hydrogen isotope ratio of urinary steroids in the context of sports drug testing.

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Serval ID
serval:BIB_1FA31B8D896A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effect of changes in the deuterium content of drinking water on the hydrogen isotope ratio of urinary steroids in the context of sports drug testing.
Journal
Analytical and Bioanalytical Chemistry
Author(s)
Piper T., Degenhardt K., Federherr E., Thomas A., Thevis M., Saugy M.
ISSN
1618-2650 (Electronic)
Publication state
Published
Issued date
11/2013
Peer-reviewed
Oui
Volume
405
Number
9
Pages
2911-2921
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Abstract
The hydrogen isotope ratio (HIR) of body water and, therefore, of all endogenously synthesized compounds in humans, is mainly affected by the HIR of ingested drinking water. As a consequence, the entire organism and all of its synthesized substrates will reflect alterations in the isotope ratio of drinking water, which depends on the duration of exposure. To investigate the effect of this change on endogenous urinary steroids relevant to doping-control analysis the hydrogen isotope composition of potable water was suddenly enriched from -50 to 200 0/00 and maintained at this level for two weeks for two individuals. The steroids under investigation were 5β-pregnane-3α,20α-diol, 5α-androst-16-en-3α-ol, 3α-hydroxy-5α-androstan-17-one (ANDRO), 3α-hydroxy-5β-androstan-17-one (ETIO), 5α-androstane-3α,17β-diol, and 5β-androstane-3α,17β-diol (excreted as glucuronides) and ETIO, ANDRO and 3β-hydroxyandrost-5-en-17-one (excreted as sulfates). The HIR of body water was estimated by determination of the HIR of total native urine, to trace the induced changes. The hydrogen in steroids is partly derived from the total amount of body water and cholesterol-enrichment could be calculated by use of these data. Although the sum of changes in the isotopic composition of body water was 150 0/00, shifts of approximately 30 0/00 were observed for urinary steroids. Parallel enrichment in their HIR was observed for most of the steroids, and none of the differences between the HIR of individual steroids was elevated beyond recently established thresholds. This finding is important to sports drug testing because it supports the intended use of this novel and complementary methodology even in cases where athletes have drunk water of different HIR, a plausible and, presumably, inevitable scenario while traveling.
Keywords
Adult, Cholesterol/urine, Deuterium/analysis, Drinking, Drinking Water/analysis, Gas Chromatography-Mass Spectrometry/methods, Humans, Hydrogen/analysis, Male, Steroids/urine, Substance Abuse Detection/methods
Pubmed
Web of science
Open Access
Yes
Create date
12/02/2013 17:21
Last modification date
01/10/2019 7:17
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