A bovine model of a respiratory Parachlamydia acanthamoebae infection.

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Ressource 1Download: serval:BIB_1F9722BE9B0D.P001 (2330.24 [Ko])
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Serval ID
serval:BIB_1F9722BE9B0D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A bovine model of a respiratory Parachlamydia acanthamoebae infection.
Journal
Pathogens and Disease
Author(s)
Lohr M., Prohl A., Ostermann C., Liebler-Tenorio E., Schroedl W., Aeby S., Greub G., Reinhold P.
ISSN
2049-632X (Electronic)
ISSN-L
2049-632X
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
73
Number
1
Pages
1-14
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
The aim of this study was to evaluate the pathogenicity of Parachlamydia (P.) acanthamoebae as a potential agent of lower respiratory tract disease in a bovine model of induced lung infection. Intrabronchial inoculation with P. acanthamoebae was performed in healthy calves aged 2-3 months using two challenge doses: 10(8) and 10(10) bacteria per animal. Controls received 10(8) heat-inactivated bacteria. Challenge with 10(8) viable Parachlamydia resulted in a mild degree of general indisposition, whereas 10(10) bacteria induced a more severe respiratory illness becoming apparent 1-2 days post inoculation (dpi), affecting 9/9 (100%) animals and lasting for 6 days. The extent of macroscopic pulmonary lesions was as high as 6.6 (6.0)% [median (range)] of lung tissue at 2-4 dpi and correlated with parachlamydial genomic copy numbers detected by PCR, and with bacterial load estimated by immunohistochemistry in lung tissue. Clinical outcome, acute phase reactants, pathological findings and bacterial load exhibited an initial dose-dependent effect on severity. Animals fully recovered from clinical signs of respiratory disease within 5 days. The bovine lung was shown to be moderately susceptible to P. acanthamoebae, exhibiting a transient pneumonic inflammation after intrabronchial challenge. Further studies are warranted to determine the precise pathophysiologic pathways of host-pathogen interaction.
Pubmed
Web of science
Open Access
Yes
Create date
19/05/2015 18:30
Last modification date
25/09/2019 7:08
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