Superoxide anions induce the maturation of human dendritic cells.

Details

Serval ID
serval:BIB_1ECC715BACF9
Type
Article: article from journal or magazin.
Collection
Publications
Title
Superoxide anions induce the maturation of human dendritic cells.
Journal
American Journal of Respiratory and Critical Care Medicine
Author(s)
Kantengwa S., Jornot L., Devenoges C., Nicod L.P.
ISSN
1073-449X[print], 1073-449X[linking]
Publication state
Published
Issued date
2003
Volume
167
Number
3
Pages
431-437
Language
english
Abstract
Dendritic cells play a key role in immune responses. There is growing evidence that reactive oxygen species participate in signaling pathways involving nuclear factor (NF)-kappaB, leading to expression of important immune system genes. We found that, unlike H2O2, reactive oxygen species generated by the reaction of oxidase on xanthine induced early phenotypic maturation of dendritic cells by upregulating specific markers CD80, CD83, and CD86 and downregulating mannose receptor-mediated endocytosis. Maturation induced by xanthine oxidase was prevented by allopurinol, an inhibitor of xanthine oxidase activity, and by N-acetylcysteine. The proteasome inhibitor MG-132, which blocks NF-kappaB activation, also inhibited CD86 upregulation, but not endocytosis downregulation by reactive oxygen species. Finally, xanthine-xanthine oxidase enhanced or blocked antigen presentation by dendritic cells depending on whether they had been prepulsed or not with the antigen. Taken together, these results demonstrate that oxidative stress induces phenotypic and functional maturation of dendritic cells, partly through an NF-kappaB-dependent mechanism.
Keywords
Cells, Cultured, Dendritic Cells/drug effects, Dendritic Cells/physiology, Humans, Reactive Oxygen Species/immunology, Superoxides/immunology, Xanthine/antagonists & inhibitors, Xanthine/pharmacology, Xanthine Oxidase/antagonists & inhibitors, Xanthine Oxidase/pharmacology
Pubmed
Web of science
Create date
19/02/2010 20:07
Last modification date
03/03/2018 14:36
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