Thrombin in ischemic neuronal death

Details

Serval ID
serval:BIB_1EB2BE6DEA93
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Thrombin in ischemic neuronal death
Journal
Experimental Neurology
Author(s)
de Castro Ribeiro  M., Badaut  J., Price  M., Meins  M., Bogousslavsky  J., Monard  D., Hirt  L.
ISSN
0014-4886 (Print)
Publication state
Published
Issued date
03/2006
Peer-reviewed
Oui
Volume
198
Number
1
Pages
199-203
Notes
Comparative Study In Vitro Journal Article Research Support, Non-U.S. Gov't --- Old month value: Mar
Abstract
Thrombin plays a role in cerebral ischemia as rats subjected to focal cerebral ischemia were protected by the intracerebral injection of hirudin, a selective thrombin inhibitor. To separate the roles of thrombin in cell death and in coagulation, we have used an in vitro approach to test the effect of hirudin and of protease nexin-1 (PN-1), a cerebral thrombin inhibitor, on neuronal ischemia. Rat organotypic hippocampal slice cultures were subjected to oxygen (5%) and glucose (1 mmol/L) deprivation (OGD) during 30 min. Hirudin or PN-1 administered after OGD significantly prevented neuronal death in the CA1 region. After 24 h, there was a marked increase in thrombin immunoreactivity on Western blots. Thrombin therefore contributes to ischemic damage in neural tissue in vitro.
Keywords
Animals Animals, Newborn Anoxia/complications/drug therapy Blotting, Western/methods Cell Death/drug effects/physiology Disease Models, Animal Dose-Response Relationship, Drug Gene Expression Regulation/drug effects Glucose/deficiency Hippocampus/pathology Hirudin Therapy/methods Hirudins/pharmacology Ischemia/etiology/metabolism/*pathology/prevention & control Neurons/drug effects/metabolism Protease Inhibitors/pharmacology Rats Rats, Sprague-Dawley Thrombin/antagonists & inhibitors/*physiology
Pubmed
Web of science
Create date
25/01/2008 12:40
Last modification date
20/08/2019 12:54
Usage data