Endothelial nitric oxide synthase gene transfer restores endothelium-dependent relaxations and attenuates lesion formation in carotid arteries in apolipoprotein E-deficient mice.

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Serval ID
serval:BIB_1DCEAB12AC5F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Endothelial nitric oxide synthase gene transfer restores endothelium-dependent relaxations and attenuates lesion formation in carotid arteries in apolipoprotein E-deficient mice.
Journal
Basic research in cardiology
Author(s)
Mujynya-Ludunge K., Viswambharan H., Driscoll R., Ming X.F., von Segesser L.K., Kappenberger L., Yang Z., Vassalli G.
ISSN
1435-1803[electronic]
Publication state
Published
Issued date
2005
Volume
100
Number
2
Pages
102-11
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
Nitric oxide (NO) and monocyte chemoattractant protein-1 (MCP-1) exert partly opposing effects in vascular biology. NO plays pleiotropic vasoprotective roles including vasodilation and inhibition of platelet aggregation, smooth muscle cell proliferation, and endothelial monocyte adhesion, the last effect being mediated by MCP-1 downregulation. Early stages of arteriosclerosis are associated with reduced NO bioactivity and enhanced MCP-1 expression. We have evaluated adenovirus-mediated gene transfer of human endothelial NO synthase (eNOS) and of a N-terminal deletion (8ND) mutant of the MCP-1 gene that acts as a MCP-1 inhibitor in arteriosclerosis-prone, apolipoprotein E-deficient (ApoE(-/-)) mice. Endothelium-dependent relaxations were impaired in carotid arteries instilled with a noncoding adenoviral vector but were restored by eNOS gene transfer (p < 0.01). A perivascular collar was placed around the common carotid artery to accelerate lesion formation. eNOS gene transfer reduced lesion surface areas, intima/media ratios, and macrophage contents in the media at 5-week follow-up (p < 0.05). In contrast, 8ND-MCP-1 gene transfer did not prevent lesion formation. In conclusion, eNOS gene transfer restores endothelium-dependent vasodilation and inhibits lesion formation in ApoE(-/-) mouse carotids. Further studies are needed to assess whether vasoprotection is maintained at later disease stages and to evaluate the long-term efficacy of eNOS gene therapy for primary arteriosclerosis.
Keywords
Adenoviridae, Animals, Apolipoproteins E, Carotid Artery Diseases, Carotid Artery, Common, Chemokine CCL2, Cholesterol, Cholesterol, HDL, Dietary Fats, Disease Models, Animal, Dose-Response Relationship, Drug, Gene Therapy, Gene Transfer Techniques, Genetic Vectors, Humans, Macrophages, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Mutation, Nitric Oxide Synthase Type III, Vasodilation, Vasodilator Agents
Pubmed
Web of science
Open Access
Yes
Create date
15/02/2008 12:28
Last modification date
01/10/2019 7:17
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