Group-specific, major histocompatibility complex class I-restricted cytotoxic responses to human immunodeficiency virus 1 (HIV-1) envelope proteins by cloned peripheral blood T cells from an HIV-1-infected individual
Details
Serval ID
serval:BIB_1DB8D4B6E004
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Group-specific, major histocompatibility complex class I-restricted cytotoxic responses to human immunodeficiency virus 1 (HIV-1) envelope proteins by cloned peripheral blood T cells from an HIV-1-infected individual
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424 (Print)
Publication state
Published
Issued date
11/1988
Volume
85
Number
22
Pages
8638-42
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
Freshly separated unfractionated peripheral blood mononuclear cells (PBMC) and cloned cell lines from a healthy human immunodeficiency virus 1 (HIV-1)-seropositive individual were examined for cytotoxic responses to HIV proteins expressed by recombinant vaccinia viruses. It was found that freshly isolated PBMC recognize variant envelope proteins of HIV-1 but not a more distantly related envelope protein derived from the simian immunodeficiency virus (SIVmac). Although the effector cells were predominantly CD8+, both MHC-matched and -unmatched target cells were lysed. Cytotoxic T lymphocyte (CTL) clones were found to lyse cells expressing HIV-1 envelope or reverse transcriptase. In contrast to the cytotoxic response detected with PBMC, the cloned CTLs were major histocompatibility complex (MHC) class I restricted. Our finding that a cloned CTL line lysed cells expressing highly divergent HIV envelopes strongly suggested that a conserved epitope was recognized. Identification of these shared epitopes may assist in designing a vaccine for HIV-1 that could stimulate MHC-restricted cytotoxic responses.
Keywords
Acquired Immunodeficiency Syndrome/*immunology
Cells, Cultured
Clone Cells
*Cytotoxicity, Immunologic
*Genes, MHC Class I
HIV-1/*immunology
Humans
RNA-Directed DNA Polymerase/analysis
T-Lymphocytes/*immunology
Vaccinia virus/immunology
Viral Envelope Proteins/*immunology
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 15:13
Last modification date
20/08/2019 12:54