Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains.

Details

Serval ID
serval:BIB_1CEF81B19652
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains.
Journal
Frontiers in neuroscience
Author(s)
Hung S.T., Llobet Rosell A., Jurriens D., Siemons M., Soloviev O., Kapitein L.C., Grußmayer K., Neukomm L.J., Verhaegen M., Smith C.
ISSN
1662-4548 (Print)
ISSN-L
1662-453X
Publication state
Published
Issued date
2022
Peer-reviewed
Oui
Volume
16
Pages
954949
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence decreases the localization precision. Here, we synergistically combine sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations. We apply AO-SOLEIL on several dSTORM samples including brains of adult Drosophila. We observed a 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction while we used synergistic solution. AO-SOLEIL enhances the overall imaging resolution and further facilitates the visualization of sub-cellular structures in tissue.
Keywords
Drosophila, Super-resolution Microscopy, adaptive optics, brain, localization microscopy
Pubmed
Web of science
Open Access
Yes
Create date
02/11/2022 8:39
Last modification date
11/11/2022 6:39
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