Efficiency of adeno-associated virus type-2 vectors in non-human primate Schwann cells.

Details

Serval ID
serval:BIB_1C48BB938D65
Type
Article: article from journal or magazin.
Collection
Publications
Title
Efficiency of adeno-associated virus type-2 vectors in non-human primate Schwann cells.
Journal
Neuroreport
Author(s)
Girard C., Tenenbaum L., Chtarto A., Attali B., Salvetti A., Bachelin C., Evercooren A.B., Lachapelle F.
ISSN
0959-4965 (Print)
ISSN-L
0959-4965
Publication state
Published
Issued date
2005
Volume
16
Number
16
Pages
1757-1762
Language
english
Abstract
Adult macaque Schwann cells were infected using adeno-associated virus type-2-derived vectors expressing the green fluorescent protein reporter gene under the control of the cytomegalovirus, the hybrid cytomegalovirus-betaactin, the myelin basic protein or the tetracycline-inducible promoters. On the basis of green fluorescent protein expression, gene transfer efficiency was compared in resting and dividing conditions following or not following hydroxyurea or etoposide treatment. Hydroxyurea allowed promoter-dependent expression of green fluorescent protein in infected Schwann cells. Etoposide treatment led to a high percentage of green fluorescent protein expressing cells (over 50%) with all promoters tested. When infected cells were grafted into demyelinated nude mice spinal cord, green fluorescent protein expression was only observed with the cytomegalovirus-betaactin and tetracycline-inducible promoters. In addition, adeno-associated virus type-2 infection reduced the grafted cell survival but increased their differentiation.
Keywords
Analysis of Variance, Animals, Cell Count/methods, Cell Proliferation/drug effects, Cell Transplantation/methods, Cells, Cultured, Cytomegalovirus/physiology, Demyelinating Diseases/chemically induced, Demyelinating Diseases/surgery, Dependovirus/physiology, Etoposide/pharmacology, Fibroblasts/metabolism, Fibroblasts/virology, Gene Expression Regulation/drug effects, Gene Expression Regulation/physiology, Genetic Vectors/physiology, Green Fluorescent Proteins/metabolism, Hydroxyurea/pharmacology, Immunohistochemistry/methods, Macaca fascicularis, Mice, Myelin Basic Proteins/metabolism, Nucleic Acid Synthesis Inhibitors/pharmacology, Promoter Regions, Genetic/drug effects, Promoter Regions, Genetic/physiology, Schwann Cells/metabolism, Schwann Cells/virology, Time Factors, Transduction, Genetic
Pubmed
Web of science
Create date
01/04/2011 10:10
Last modification date
20/08/2019 12:52
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