Adjuvant activity of polymer microparticles and Montanide ISA 720 on immune responses to Plasmodium falciparum MSP2 long synthetic peptides in mice

Details

Serval ID
serval:BIB_1BF14D51B8E5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Adjuvant activity of polymer microparticles and Montanide ISA 720 on immune responses to Plasmodium falciparum MSP2 long synthetic peptides in mice
Journal
Vaccine
Author(s)
Mata  E., Carcaboso  A. M., Hernandez  R. M., Igartua  M., Corradin  G., Pedraz  J. L.
ISSN
0264-410X (Print)
Publication state
Published
Issued date
01/2007
Volume
25
Number
5
Pages
877-85
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 15
Abstract
The purpose of this work was to test the immunogenicity in C57BL mice of two synthetic peptides derived from the constant region of 3D7 and FC27 Plasmodium falciparum MSP2 dimorphic proteins, either microencapsulated into poly-lactide-co-glycolide acid microparticles (PLGA MP) or delivered with the human compatible adjuvant Montanide ISA 720 for comparison. Potent and prolonged antibody responses were obtained for both peptides by using PLGA MP formulations after subcutaneous or intradermal injections. As compared to the subcutaneous route of immunization, the intradermal route induced greater immune responses. Montanide adjuvant was effective in eliciting antibodies against the 3D7 peptide but not against the FC27 peptide. Peptide-specific cytophilic antibodies (IgG2a) were detected after boosting with homologous peptide for all vaccine formulations. MP formulations elicited a lower IgE secretion as compared to that observed for both Montanide formulated vaccines. Our results demonstrate the ability of the polymer microparticles to overcome the lack of immunogenicity of FC27 MSP2 peptide in C57BL mice and their potential to induce desirable immune responses against malaria.
Keywords
Adjuvants, Immunologic/*administration & dosage Amino Acid Sequence Animals Antibodies, Protozoan/blood Antigens, Protozoan/*immunology Cross Reactions Female Immunoglobulin A/blood Immunoglobulin G/blood/classification Lactic Acid/*administration & dosage Malaria Vaccines/*administration & dosage/immunology Mannitol/administration & dosage/*analogs & derivatives Mice Mice, Inbred C57BL Microspheres Molecular Sequence Data Oleic Acids/*administration & dosage Peptide Fragments/*immunology Plasmodium falciparum/*immunology Polyglycolic Acid/*administration & dosage Polymers/*administration & dosage Protozoan Proteins/*immunology Vaccines, Synthetic/*administration & dosage/immunology
Pubmed
Web of science
Create date
24/01/2008 15:55
Last modification date
20/08/2019 13:52
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