FITC-albumin as a marker for assessment of endothelial permeability in mice: comparison with 125I-albumin

Details

Serval ID
serval:BIB_1B84A5301DD3
Type
Article: article from journal or magazin.
Collection
Publications
Title
FITC-albumin as a marker for assessment of endothelial permeability in mice: comparison with 125I-albumin
Journal
Exp Lung Res
Author(s)
Boutoille D., Marechal X., Pichenot M., Chemani C., Guery B., Faure K.
ISSN
1521-0499 (Electronic)
ISSN-L
0190-2148
Publication state
Published
Issued date
05/2009
Volume
35
Number
4
Pages
263-71
Language
english
Notes
Boutoille, D
Marechal, X
Pichenot, Marie
Chemani, C
Guery, B
Faure, K
eng
Comparative Study
Evaluation Study
England
Exp Lung Res. 2009 May;35(4):263-71. doi: 10.1080/01902140802632290.
Abstract
Transvascular transport of labeled-albumin is used to study endothelial permeability in experimental murine models of pulmonary infections. But radio-tagged albumin necessitates heavy safety procedures in terms of storage, manipulation and evacuation. The authors tested fluorescein isothiocyanate-tagged albumin (FITC-albumin) as a new marker for determination of endothelial permeability in a murine model of lung infection by Pseudomonas aeruginosa PAO1, in comparison with a standard method with (125)I-albumin. The mean permeability +/- SEM measured with (125)I-albumin was 2.45%/2 h +/- 0.37 for the control mice and 6.65%/2 h +/- 0.77 for the infected ones (P < .0001). With FITC-albumin, results obtained for both groups were respectively 4.96%/2 h +/- 0.64 and 11.5%/2 h +/- 1.2 (P < .0001). Spearman's rank coefficient was equal to .88 (P < .0001), showing a very strong correlation between both methods of measurement. The Bland-Altman analysis of bias revealed that there was no significant bias between FITC-albumin-derived and (125)I-albumin-derived values. The correction of the values obtained in plasma and lung homogenate supernatants by the subtraction of natural spontaneous fluorescence measured in these samples was crucial for the calculation of endothelial permeability in this new method. We believe that FITC-albumin can be useful for assessment of endothelial permeability in murine models of pulmonary diseases.
Keywords
Animals, *Capillary Permeability, Endothelium, Vascular/*metabolism, Fluorescein-5-isothiocyanate/*analogs & derivatives/pharmacokinetics, Iodine Radioisotopes/pharmacokinetics, Methods, Mice, Pseudomonas aeruginosa, Reproducibility of Results, Respiratory Tract Infections/diagnosis/pathology, *Serum Albumin/pharmacokinetics
Pubmed
Create date
29/04/2021 10:59
Last modification date
30/04/2021 6:38
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