Rapid, combinatorial analysis of membrane compartments in intact plants with a multicolor marker set.

Details

Serval ID
serval:BIB_1B448D5FC175
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Rapid, combinatorial analysis of membrane compartments in intact plants with a multicolor marker set.
Journal
Plant Journal
Author(s)
Geldner N., Dénervaud-Tendon V., Hyman D.L., Mayer U., Stierhof Y.D., Chory J.
ISSN
1365-313X[electronic], 0960-7412[linking]
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
59
Number
1
Pages
169-178
Language
english
Abstract
Plant membrane compartments and trafficking pathways are highly complex, and are often distinct from those of animals and fungi. Progress has been made in defining trafficking in plants using transient expression systems. However, many processes require a precise understanding of plant membrane trafficking in a developmental context, and in diverse, specialized cell types. These include defense responses to pathogens, regulation of transporter accumulation in plant nutrition or polar auxin transport in development. In all of these cases a central role is played by the endosomal membrane system, which, however, is the most divergent and ill-defined aspect of plant cell compartmentation. We have designed a new vector series, and have generated a large number of stably transformed plants expressing membrane protein fusions to spectrally distinct, fluorescent tags. We selected lines with distinct subcellular localization patterns, and stable, non-toxic expression. We demonstrate the power of this multicolor 'Wave' marker set for rapid, combinatorial analysis of plant cell membrane compartments, both in live-imaging and immunoelectron microscopy. Among other findings, our systematic co-localization analysis revealed that a class of plant Rab1-homologs has a much more extended localization than was previously assumed, and also localizes to trans-Golgi/endosomal compartments. Constructs that can be transformed into any genetic background or species, as well as seeds from transgenic Arabidopsis plants, will be freely available, and will promote rapid progress in diverse areas of plant cell biology.
Keywords
Cell Compartmentation, Gene Expression Regulation, Plant, Genetic Vectors, Luminescent Proteins/genetics, Luminescent Proteins/metabolism, Microscopy, Immunoelectron, Plant Proteins/genetics, Plant Proteins/metabolism, Plants, Genetically Modified/genetics, Plants, Genetically Modified/metabolism, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/metabolism, Transformation, Genetic
Pubmed
Web of science
Create date
13/01/2010 12:02
Last modification date
20/08/2019 12:52
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