Cytochemical and immunocytochemical characterization of nuclear bodies during hibernation.

Details

Serval ID
serval:BIB_1B16B499F64F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cytochemical and immunocytochemical characterization of nuclear bodies during hibernation.
Journal
European Journal of Cell Biology
Author(s)
Malatesta M., Zancanaro C., Martin T.E., Chan E.K., Amalric F., Lührmann R., Vogel P., Fakan S.
ISSN
0171-9335 (Print)
ISSN-L
0171-9335
Publication state
Published
Issued date
1994
Volume
65
Number
1
Pages
82-93
Language
english
Abstract
Brown adipose tissue and liver of hibernating, arousing and euthermic individuals of the dormouse Muscardinus avellanarius were studies using ultrastructural cytochemistry and immunocytochemistry with the aim to investigate possible fine structural modifications of the cell nucleus during the seasonal cycle. The general morphology of brown adipocyte and hepatocyte nuclei was similar in the three experimental groups. However, three nuclear structural constituents were identified only in hibernating individuals: coiled bodies (CBs) and amorphous bodies (ABs) were observed in hepatocytes and, together with bundles of nucleoplasmic fibrils (NF), were present in brown adipocytes of hibernating dormice. In arousing animals only some structural constituents suggestive of poorly structured CBs were found. The latter showed the same immunocytochemical features as CBs of hibernating individuals, suggesting that they are disappearing CBs. A possible involvement of CBs in storing and/or processing RNA which must be rapidly and abundantly released upon arousal is discussed. ABs similarly to CBs contain RNA and nucleoplasmic ribonucleoproteins (RNPs) and could also be involved in mRNA pathways. NF do not contain nucleic acids or RNPs and seem to be composed of protein-aceous material; their functional role in the nuclear metabolism of hibernating brown adipocytes remains unclear.
Keywords
Adipocytes/chemistry, Adipocytes/ultrastructure, Adipose Tissue, Brown/chemistry, Adipose Tissue, Brown/cytology, Animals, Arousal/physiology, Cell Nucleus/chemistry, Cell Nucleus/ultrastructure, Hibernation/physiology, Histocytochemistry, Immunohistochemistry, Liver/chemistry, Liver/ultrastructure, Microscopy, Electron, Rodentia/anatomy & histology, Rodentia/metabolism
Pubmed
Web of science
Create date
24/01/2008 17:33
Last modification date
20/08/2019 12:51
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