A two-compartment mathematical model of neuroglial metabolism using [1-(11)C] acetate.

Details

Serval ID
serval:BIB_1A57E68EDDA4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A two-compartment mathematical model of neuroglial metabolism using [1-(11)C] acetate.
Journal
Journal of Cerebral Blood Flow and Metabolism
Author(s)
Lanz B., Uffmann K., T Wyss M., Weber B., Buck A., Gruetter R.
ISSN
1559-7016 (Electronic)
ISSN-L
0271-678X
Publication state
Published
Issued date
2012
Volume
32
Number
3
Pages
548-559
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
The purpose of this study was to develop a two-compartment metabolic model of brain metabolism to assess oxidative metabolism from [1-(11)C] acetate radiotracer experiments, using an approach previously applied in (13)C magnetic resonance spectroscopy (MRS), and compared with an one-tissue compartment model previously used in brain [1-(11)C] acetate studies. Compared with (13)C MRS studies, (11)C radiotracer measurements provide a single uptake curve representing the sum of all labeled metabolites, without chemical differentiation, but with higher temporal resolution. The reliability of the adjusted metabolic fluxes was analyzed with Monte-Carlo simulations using synthetic (11)C uptake curves, based on a typical arterial input function and previously published values of the neuroglial fluxes V(tca)(g), V(x), V(nt), and V(tca)(n) measured in dynamic (13)C MRS experiments. Assuming V(x)(g)=10 × V(tca)(g) and V(x)(n)=V(tca)(n), it was possible to assess the composite glial tricarboxylic acid (TCA) cycle flux V(gt)(g) (V(gt)(g)=V(x)(g) × V(tca)(g)/(V(x)(g)+V(tca)(g))) and the neurotransmission flux V(nt) from (11)C tissue-activity curves obtained within 30 minutes in the rat cortex with a beta-probe after a bolus infusion of [1-(11)C] acetate (n=9), resulting in V(gt)(g)=0.136±0.042 and V(nt)=0.170±0.103 μmol/g per minute (mean±s.d. of the group), in good agreement with (13)C MRS measurements.
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Create date
24/03/2012 9:39
Last modification date
20/08/2019 12:51
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