First annotated draft genomes of non-marine ostracods (Ostracoda, Crustacea) with different reproductive modes.

Details

Serval ID
serval:BIB_19612ACE0AC4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
First annotated draft genomes of non-marine ostracods (Ostracoda, Crustacea) with different reproductive modes.
Journal
G3
Author(s)
Tran Van P., Anselmetti Y., Bast J., Dumas Z., Galtier N., Jaron K.S., Martens K., Parker D.J., Robinson-Rechavi M., Schwander T., Simion P., Schön I.
ISSN
2160-1836 (Electronic)
ISSN-L
2160-1836
Publication state
In Press
Peer-reviewed
Oui
Language
english
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Abstract
Ostracods are one of the oldest crustacean groups with an excellent fossil record and high importance for phylogenetic analyses but genome resources for this class are still lacking. We have successfully assembled and annotated the first reference genomes for three species of non-marine ostracods; two with obligate sexual reproduction (Cyprideis torosa and Notodromas monacha) and the putative ancient asexual Darwinula stevensoni. This kind of genomic research has so far been impeded by the small size of most ostracods and the absence of genetic resources such as linkage maps or BAC libraries that were available for other crustaceans. For genome assembly, we used an Illumina-based sequencing technology, resulting in assemblies of similar sizes for the three species (335-382Mb) and with scaffold numbers and their N50 (19-56 kb) in the same orders of magnitude. Gene annotations were guided by transcriptome data from each species. The three assemblies are relatively complete with BUSCO scores of 92-96. The number of predicted genes (13,771-17,776) is in the same range as Branchiopoda genomes but lower than in most malacostracan genomes. These three reference genomes from non-marine ostracods provide the urgently needed basis to further develop ostracods as models for evolutionary and ecological research.
Keywords
Cyprideis torosa, Darwinula stevensoni, ancient asexual, sexual, Cyprideis torosa, Darwinula stevensoni
Pubmed
Open Access
Yes
Create date
22/02/2021 9:14
Last modification date
02/03/2021 6:25
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