Circulating microRNAs as long-term biomarkers for the detection of erythropoiesis-stimulating agent abuse.

Details

Serval ID
serval:BIB_1956234662DB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Circulating microRNAs as long-term biomarkers for the detection of erythropoiesis-stimulating agent abuse.
Journal
Drug Testing and Analysis
Author(s)
Leuenberger N., Jan N., Pradervand S., Robinson N., Saugy M.
ISSN
1942-7603
Publication state
Published
Issued date
2011
Volume
3
Number
11-12
Pages
771-776
Language
english
Abstract
MicroRNAs (miRNAs) are small, non-protein coding transcripts involved in many cellular and physiological mechanisms. Recently, a new class of miRNA called 'circulating miRNAs' was found in cell-free body fluids such as plasma and urine. Circulating miRNAs have been shown to be very stable, specific, and sensitive biomarkers. In this paper, we investigate whether circulating miRNAs can serve as biomarkers for erythropoiesis-stimulating agent abuse. To this end, we analyzed miRNA levels in plasma by miRNA microarrays and quantitative real-time polymerase chain reaction (PCR). Plasma samples are derived from a clinical study with healthy subjects injected with erythropoiesis-stimulating agent (C.E.R.A.). Based on microarray results, we observed a significant difference in the levels of miRNAs in plasma after C.E.R.A. injection. We demonstrated that a specific miRNA, miR-144, exhibit a high increase that lasts 27 days after C.E.R.A. stimulation. Considering the fact that miR-144 is an essential erythropoiesis agent in different organisms, these findings suggest the possibility of using miR-144 as a sensitive and informative biomarker to detect C.E.R.A. abuse. Copyright © 2011 John Wiley & Sons, Ltd.
Pubmed
Web of science
Create date
06/01/2012 14:49
Last modification date
20/08/2019 13:50
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